Isolation and characterization of microsatellite loci in Ononis repens, Leguminosae

被引：5

作者：

Kloda, JM

Dean, PDG

MacDonald, D

Mayes, S

机构：

[1] Univ Cambridge, Dept Genet, Cambridge CB2 3EH, England

[2] Cambio Ltd, Irwin Ctr, Cambridge CB3 8AR, England

[3] Univ Nottingham, Sch Biosci, Loughborough LE12 5RD, Leics, England

来源：

MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 04期

关键词：

microsatellite; Ononis; restharrow;

D O I：

10.1111/j.1471-8286.2004.00748.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ten polymorphic microsatellites were isolated from Ononis repens, using a microsatellite enrichment protocol and selective hybridization with a mixed synthetic dinucleotide repeat probe. Primers for DNA amplifications using polymerase chain reaction (PCR) were designed and synthesized. The loci amplified in both O. repens and O. spinosa. All 10 loci were polymorphic in each of four populations studied. These microsatellite markers will be useful for population genetic analysis and biodiversity studies of O. repens and O. spinosa.

引用

页码：596 / 598

页数：3

共 6 条

[1]

Dellaporta S. L., 1983, Plant Molecular Biology Reporter, V1, P19, DOI [10.1007/BF02712670, DOI 10.1007/BF02712670]

[2]

Edwards KJ, 1996, BIOTECHNIQUES, V20, P758

[3]

MORISSET P, 1978, SERIES VULGARES SIRJ, V12, P145

[4] GENEPOP (VERSION-1.2) - POPULATION-GENETICS SOFTWARE FOR EXACT TESTS AND ECUMENICISM [J].

RAYMOND, M ;

ROUSSET, F .

JOURNAL OF HEREDITY, 1995, 86 (03) :248-249

[5]

Rozen S, 2000, Methods Mol Biol, V132, P365

[6] Construction and characterization of a large-fragment chicken bacterial artificial chromosome library [J].

Zimmer, R ;

Gibbins, AMV .

GENOMICS, 1997, 42 (02) :217-226

← 1 →