Nanoindentation Methods to Measure Viscoelastic Properties of Single Cells Using Sharp, Flat, and Buckling Tips Inside ESEM

被引：39

作者：

Ahmad, Mohd Ridzuan ^{[1
,2
]}

Nakajima, Masahiro ^{[1
]}

Kojima, Seiji ^{[3
]}

Homma, Michio ^{[3
]}

Fukuda, Toshio ^{[1
]}

机构：

[1] Nagoya Univ, Dept Micro Nano Syst Engn, Nagoya, Aichi 4648603, Japan

[2] Univ Teknol Malaysia, Skudai 81310, Malaysia

[3] Nagoya Univ, Grad Sch Sci, Div Biol Sci, Nagoya, Aichi 4648602, Japan

来源：

IEEE TRANSACTIONS ON NANOBIOSCIENCE | 2010年 / 9卷 / 01期

关键词：

Environmental-SEM (ESEM); nanomanipulation; single cell analysis; viscoelasticity; yeast cells; MECHANICAL-PROPERTIES; AXISYMMETRICAL INDENTERS; SACCHAROMYCES-CEREVISIAE; YEAST SUSPENSIONS; FORCE MICROSCOPE; CREEP COMPLIANCE; CONTACT DEPTH; INDENTATION; SOLIDS; STIFFNESS;

D O I：

10.1109/TNB.2009.2034849

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper, methods to measure viscoelastic properties of time-dependent materials are proposed using sharp, flat, and buckling tips inside an environmental SEM. Single W303 yeast cells were employed in this study. Each of the tips was used to indent single cells in a nanoindentation test. Three loading histories were used: 1) a ramp loading history, in which a sharp indenter was used; 2) a step loading history, in which a flat indenter was implemented; and 3) a fast unloading history, in which a buckling nanoneedle was applied. Analysis of the viscoelastic properties of single cells was performed for each of the loading histories by choosing an appropriate theory between the correspondence principle and the functional equation. Results from each of the tests show good agreement, from which strong conclusion can be drawn.

引用

页码：12 / 23

页数：12

共 36 条

[1]

AHMAD MR, 2007, P 2007 IEEE RSJ INT, P596

[2]

Cheng L, 2000, J POLYM SCI POL PHYS, V38, P10, DOI 10.1002/(SICI)1099-0488(20000101)38:1<10::AID-POLB2>3.0.CO

[3]

2-6

[4] Spherical-tip indentation of viscoelastic material [J].

Cheng, L ;

Xia, X ;

Scriven, LE ;

Gerberich, WW .

MECHANICS OF MATERIALS, 2005, 37 (01) :213-226

[5] Methods of obtaining instantaneous modulus of viscoelastic solids using displacement-controlled instrumented indentation with axisymmetric indenters of arbitrary smooth profiles [J].

Cheng, Yang-Tse ;

Cheng, Che-Min ;

Ni, Wangyang .

MATERIALS SCIENCE AND ENGINEERING A-STRUCTURAL MATERIALS PROPERTIES MICROSTRUCTURE AND PROCESSING, 2006, 423 (1-2) :2-7

[6] Relationships between initial unloading slope, contact depth, and mechanical properties for spherical indentation in linear viscoelastic solids [J].

Cheng, YT ;

Cheng, CM .

MATERIALS SCIENCE AND ENGINEERING A-STRUCTURAL MATERIALS PROPERTIES MICROSTRUCTURE AND PROCESSING, 2005, 409 (1-2) :93-99

[7] Relationship between contact stiffness, contact depth, and mechanical properties for indentation in linear viscoelastic solids using axisymmetric indenters [J].

Cheng, YT ;

Cheng, CM .

STRUCTURAL CONTROL & HEALTH MONITORING, 2006, 13 (01) :561-569

[8] Determination of the spring constants of probes for force microscopy/spectroscopy [J].

Gibson, CT ;

Watson, GS ;

Myhra, S .

NANOTECHNOLOGY, 1996, 7 (03) :259-262

[9] Measuring the constitutive behavior of viscoelastic solids in the time and frequency domain using flat punch nanoindentation [J].

Herbert, E. G. ;

Oliver, W. C. ;

Lumsdaine, A. ;

Pharr, G. M. .

JOURNAL OF MATERIALS RESEARCH, 2009, 24 (03) :626-637

[10] Measurements of two independent viscoelastic functions by nanoindentation [J].

Huang, G. ;

Lu, H. .

EXPERIMENTAL MECHANICS, 2007, 47 (01) :87-98

← 1 2 3 4 →