Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer

被引：69

作者：

Frye, Michaela ^{[1
]}

Dragoni, Ilaria ^{[2
]}

Chin, Suet-Feung ^{[3
]}

Spiteri, Immaculada ^{[3
]}

Kurowski, Agata ^{[1
]}

Provenzano, Elena ^{[3
,4
,5
]}

Green, Andrew ^{[6
,7
]}

Ellis, Ian O. ^{[6
,7
]}

Grimmer, Donna ^{[1
]}

Teschendorff, Andrew ^{[3
]}

Zouboulis, Christos C. ^{[8
,9
,10
]}

Caldas, Carlos ^{[3
,4
,5
]}

Watt, Fiona M. ^{[1
,3
]}

机构：

[1] Wellcome Trust Ctr Stem Cell Res, Cambridge CB2 1QR, England

[2] CR UK Canc Res Technol Ltd, London WC2A 3NL, England

[3] Li Ka Shing Ctr, CR UK Cambridge Res Inst, Cambridge CB2 0RE, England

[4] Cambridge Breast Unit, Cambridge CB2 2SP, England

[5] NIHR Cambridge Biomed Res Ctr, Cambridge CB2 2SP, England

[6] Univ Nottingham, Sch Mol Med Sci, Div Pathol, Nottingham NG5 1PB, England

[7] Nottingham Univ Hosp NHS Trust, Nottingham NG5 1PB, England

[8] Dessau Med Ctr, Dept Dermatol, D-06847 Dessau, Germany

[9] Dessau Med Ctr, Dept Venereol, D-06847 Dessau, Germany

[10] Dessau Med Ctr, Dept Allergol & Immunol, D-06847 Dessau, Germany

来源：

CANCER LETTERS | 2010年 / 289卷 / 01期

关键词：

RNA methyltransferase; Myc; Gene amplification; Breast cancer; POLYMERASE-III TRANSCRIPTION; TRANSFER-RNA; TRANSLATION; TRANSFORMATION; PROLIFERATION; SUBTYPE; MYC;

D O I：

10.1016/j.canlet.2009.08.004

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have examined expression of the Myc target gene Misu (NSUN2) in breast cancer. There was extensive copy number gain, and increased mRNA and protein levels, of Misu in approximately one third of breast cancer cell lines and primary tumours examined, irrespective of tumour subtype. Genes on 5p15.31-33, where Misu is located, showed evolutionary synteny. siRNA-mediated knockdown of Misu reduced cell number in over half of the cell lines tested, irrespective of estrogen receptor status. We conclude that Misu is up-regulated in a substantial proportion of breast cancers and has therapeutic potential as a drug target. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：71 / 80

页数：10

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