Effect of Combined Ingestion of L-Theanine and L-Arginine for Short-Term Psychological Stress in Young Adults: A Randomized Placebo-Controlled Study

L-Theanine, the most abundant amino acid component in green tea, has anti -stress effects and refreshes the mental state. A recent study demonstrated that L-arginine, the second most abundant amino acid in green tea, might enhance the anti-stress effects of L-theanine. The aim of this study was to evaluated the effects of combined ingestion of L-theanine and L-arginine on psychological stress in humans. A randomized placebo -con-trolled trial was conducted including 120 healthy young adults (mean age 22.4 y, 63.3% female). Subjects were randomly assigned to theanine (200 mg L-theanine), combined the-anine/arginine (200 mg L-theanine, 50 mg L-arginine), or placebo groups. After consum-ing a test beverage, we administered a stress-loading test (Uchida-ICraepelin performance test) and performed salivary alpha-amylase activity (sAA) measurements to assess the phys-iological stress response at 0 min (immediately after), 5 min, and 15 min. The changes in sAA at 15 min after the stress-loading test were -2.75 (11.2) kIU/L in the theanine/argi-nine group, -0.40 (11.5) kIU/L in the theanine group, and 6.95 (18.6) kIU/L in the pla-cebo group. The values in the theanine/arginine (p=0.007) and theanine (p=0.02) groups differed significantly from those in the placebo group. However, the difference between the-anine/arginine and theanine groups, was not statistically significant (p=0.74). From this study, no clear conclusion could be drawn regarding the potentiating effect of theanine and arginine combined ingestion on anti-stress effects in human.