Cell biology through proteomics - ad astra per alia porci

被引：13

作者：

Bergeron, John J. M. ^{[1
,2
]}

Au, Catherine E. ^{[1
,2
]}

Desjardins, Michel ^{[3
,4
]}

McPherson, Peter S. ^{[5
]}

Nilsson, Tommy ^{[1
,2
]}

机构：

[1] McGill Univ, Res Inst, Ctr Hlth, Montreal, PQ H3A 1A1, Canada

[2] McGill Univ, Dept Med, Montreal, PQ H3A 1A1, Canada

[3] Univ Montreal, Dept Pathol & Biol Cellulaire, Montreal, PQ, Canada

[4] Univ Montreal, Dept Microbiol & Immunol, Montreal, PQ, Canada

[5] McGill Univ, Dept Neurol & Neurosurg, Montreal Neurol Inst, Montreal, PQ H3A 2B4, Canada

来源：

TRENDS IN CELL BIOLOGY | 2010年 / 20卷 / 06期

关键词：

CLATHRIN-MEDIATED ENDOCYTOSIS; LINKED OLIGOSACCHARIDE UNITS; COPI VESICLES; MASS-SPECTROMETRY; IGA1; IMMUNOGLOBULIN; ORGANELLE PROTEOME; CARBOHYDRATE UNITS; SECRETORY PATHWAY; COATED VESICLES; GOLGI;

D O I：

10.1016/j.tcb.2010.02.005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Isolated subcellular fractions have been instrumental in elucidating cell function. The use of such fractions for the identification and biochemical characterization of subcellular organelles, combined with cell- free systems, has provided key insights into the function and machineries of organelles, including those involved in vesicle transport, quality control and protein sorting. Despite their obvious utility, popular cell biology has come to regard in vitro-based approaches as inferior to in vivo-based approaches. Usual criticisms are contamination, nonrepresentative processes and an inability to recreate the dynamic processes seen in vivo: In a similar way, proteomics has been viewed with reservation. Despite this, and building on the tradition of in vitro-based approaches, organelle proteomics based on liquid chromatography and tandem mass-spectrometry has recently made significant contributions to cell biology, and now allows the molecular machineries of organelles to be defined with high precision.

引用

页码：337 / 345

页数：9

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