Effect of pulsed electric field on the proteolysis of cold boned beef M. Longissimus lumborum and M. Semimembranosus

被引:55
作者
Suwandy, Via [1 ]
Carne, Alan [2 ]
van de Ven, Remy [3 ]
Bekhit, Alaa El-Din A. [1 ]
Hopkins, David L. [4 ]
机构
[1] Univ Otago, Dept Food Sci, Dunedin, New Zealand
[2] Univ Otago, Dept Biochem, Dunedin, New Zealand
[3] Orange Agr Inst, NSW Dept Primary Ind, Orange, NSW 2800, Australia
[4] Ctr Red Meat & Sheep Dev, NSW Dept Primary Ind, Cowra, NSW 2794, Australia
关键词
desmin; Troponin-T; Frequency; Voltage; Ageing; Meat; TROPONIN-T DEGRADATION; MYOFIBRILLAR PROTEINS; LAMB LONGISSIMUS; MEAT; TENDERNESS; TENDERIZATION; TEMPERATURE; STIMULATION;
D O I
10.1016/j.meatsci.2014.10.011
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The effects of pulsed electric field (PEF) and ageing (3, 7,14 and 21 days) on the shear force, protein profile, and post-mortem proteolysis of beef loins (M. Longissimus lumborum, LL) and topsides (M. Semimembranosus, SM) were investigated using a range of pulsed electric field treatments [voltages (5 and 10 kV) and frequencies (20, 50, and 90 Hz)]. PEF treatment decreased the shear force of beef LL and SM muscles by up to 19%. The reduction in the shear force in the LL was not affected by the treatment intensity whereas the reduction in the SM was dependent on PEF frequency. PEF treated beef loins showed increased proteolysis, both early post-mortem and during subsequent post-mortem storage reflected by increased degradation of troponin-T and desmin. The most prominent troponin-T degradation was found in samples treated with 5 kV-90 Hz, 10 kV-20 Hz at day 3 and day 7 post-treatment in addition to 10 kV-50 Hz in subsequent post-treatment times. The degradation of desmin in PEF treated beef loins increased with ageing time. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:222 / 226
页数:5
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