Determination of gatifloxacin in human serum and urine by HPLC

被引：27

作者：

Borner, K ^{[1
]}

Hartwig, H

Lode, H

机构：

[1] Free Univ Berlin, Hosp Benjamin Franklin, Inst Clin Chem & Pathobiochem, D-12200 Berlin, Germany

[2] Free Univ Berlin, City Hosp Zehlendorf Heckeshorn, Pneumol Dept 1, D-14109 Berlin, Germany

来源：

CHROMATOGRAPHIA | 2000年 / 52卷 / Suppl 1期

关键词：

column liquid chromatography; gatifloxacin; human serum; human urine;

D O I：

10.1007/BF02493138

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and specific HPLC method for gatifloxacin applicable to serum and urine of volunteers was developed. The method can be operated unattended in over-night runs. it uses reversed-phase HPLC with fluorimetric detection. Trovafloxacin serves as internal standard. With a detection limit of 0.03 mg L-1 the method was sensitive enough to measure serum concentrations of gatifloxacin in healthy volunteers for 48 hours after a single oral dose of 400 mg. There was excellent agreement with results obtained by a microbiological assay.

引用

页码：S105 / S107

页数：3

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