Comparison of visceral adipose tissue DNA methylation and gene expression profiles in female adolescents with obesity

被引:13
|
作者
Barberio, Matthew D. [1 ]
Nadler, Evan P. [2 ,3 ,4 ]
Sevilla, Samantha [2 ]
Lu, Rosemary [2 ]
Harmon, Brennan [1 ]
Hubal, Monica J. [1 ,5 ]
机构
[1] Childrens Natl Med Ctr, Med Genet Res Ctr, Childrens Res Inst, Washington, DC 20010 USA
[2] Childrens Natl Med Ctr, Childrens Res Inst, Sheikh Zayed Inst Pediat Surg Innovat, Washington, DC 20010 USA
[3] Childrens Natl Med Ctr, Div Pediat Surg, Washington, DC 20010 USA
[4] George Washington Univ, Sch Med, Dept Integrat Syst Biol, Washington, DC USA
[5] Indiana Univ Purdue Univ, Dept Kinesiol, Indianapolis, IN 46202 USA
来源
DIABETOLOGY & METABOLIC SYNDROME | 2019年 / 11卷 / 01期
关键词
Obesity; Adipose tissue; Epigenetics; Gene expression; CARDIOVASCULAR RISK-FACTORS; CANDIDATE GENES; FAT; DIET; INFLAMMATION; METABOLISM; MICE;
D O I
10.1186/s13098-019-0494-y
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background Epigenetic changes in visceral adipose tissue (VAT) with obesity and their effects on gene expression are poorly understood, especially during emergent obesity in youth. The current study tested the hypothesis that methylation and gene expression profiles of key growth factor and inflammatory pathways are altered in VAT from obese compared to non-obese youth. Methods VAT samples from adolescent females grouped as Lean (L; n = 15; age = 15 +/- 3 years, BMI = 21.9 +/- 3.0 kg/m(2)) or Obese (Ob; n = 15, age = 16 +/- 2 years, BMI = 45.8 +/- 9.8 kg/m(2)) were collected. Global methylation (n = 20) and gene expression (N = 30) patterns were profiled via microarray and interrogated for differences between groups by ANCOVA (p < 0.05), followed by biological pathway analyses. Results Overlapping differences in methylation and gene expression in 317 genes were found in VAT from obese compared to lean groups. PI3K/AKT Signaling (p = 1.83 x 10(-6); 11/121 molecules in dataset/pathway) was significantly overrepresented in Ob VAT according to pathway analysis. Upregulations in the PI3K/AKT signaling pathway mRNAs TFAM (p = 0.03; fold change = 1.8) and PPP2R5C (p = 0.03, FC = 2.6) were confirmed via qRT-PCR. Conclusion Our analyses show obesity-related differences in DNA methylation and gene expression in visceral adipose tissue of adolescent females. Specifically, we identified methylation site/gene expression pairs differentially regulated and mapped these differences to pathways including PI3K/AKT signaling, suggesting that PI3K/AKT signaling pathway dysfunction in obesity may be driven in part by changes in DNA methylation.
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页数:12
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