Effector Memory T Cells and CD45RO+Regulatory T Cells in Metastatic vs. Non-Metastatic Lymph Nodes in Lung Cancer Patients

被引：12

作者：

Kwiecien, Iwona ^{[1
]}

Rutkowska, Elzbieta ^{[1
]}

Sokolowski, Rafal ^{[2
]}

Bednarek, Joanna ^{[2
]}

Raniszewska, Agata ^{[1
]}

Jahnz-Rozyk, Karina ^{[2
]}

Rzepecki, Piotr ^{[3
]}

Domagala-Kulawik, Joanna ^{[4
]}

机构：

[1] Mil Inst Med, Dept Internal Med & Hematol, Lab Flow Cytometry, Warsaw, Poland

[2] Mil Inst Med, Dept Internal Med Pulmonol Allergol & Clin Immunol, Warsaw, Poland

[3] Mil Inst Med, Dept Internal Med & Hematol, Warsaw, Poland

[4] Med Univ Warsaw, Dept Internal Med Pulm Dis & Allergy, Warsaw, Poland

来源：

FRONTIERS IN IMMUNOLOGY | 2022年 / 13卷

关键词：

lymph nodes; CD4+cells; CD8+cells; EBUS-TBNA; regulatory T cells; recent thymic emigrants cells; effector memory T cells; Th17-related cells; TUMOR-INFILTRATING LYMPHOCYTES; IMMUNE-RESPONSE; INTERLEUKIN-17; SUBSETS; NAIVE; DIFFERENTIATION; SUBPOPULATIONS; POPULATIONS; MACROPHAGES; MODULATION;

D O I：

10.3389/fimmu.2022.864497

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Lymphocytes play a leading role in regulation of the immune system in lung cancer patients. The recognition of T cells profile may help in prediction of effectiveness of anticancer immunotherapy. The aim of the study was to determine the dominant subpopulation of CD4+ and CD8+ lymphocytes in metastatic and non-metastatic lymph nodes (LNs) of lung cancer patients. LNs aspirates were obtained during EBUS/TBNA procedure and cells were analyzed by flow cytometry. We showed a higher percentage of CD4+ and CD8+ effector memory T cells in the metastatic than in the non-metastatic LNs (28.6 vs. 15.3% and 28.6 vs. 14.0%, p< 0.05). The proportion of CD45RO+ T regulatory cells (CD45RO+ Tregs) was higher in the metastatic LNs than in the non-metastatic ones (65.6 vs. 31%, p< 0.05). We reported the significant differences in T cell subsets depending on the lung cancer metastatic process. We observed that the effector memory T cells were predominant subpopulations in metastatic LNs. Lymphocyte profile in LNs is easy to evaluate by flow cytometry of EBUS/TBNA samples and may reflect the immune status in lung cancer.

引用

页数：13

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