Nucleosome arrays reveal the two-start organization of the chromatin fiber

被引：416

作者：

Dorigo, B

Schalch, T

Kulangara, A

Duda, S

Schroeder, RR

Richmond, TJ ^{[1
]}

机构：

[1] ETH Honggerberg, ETH Zurich, Inst Mol Biol & Biophys, CH-8093 Zurich, Switzerland

[2] Max Planck Inst Med Res, Dept Biomed Mech, D-69120 Heidelberg, Germany

来源：

SCIENCE | 2004年 / 306卷 / 5701期

关键词：

D O I：

10.1126/science.1103124

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Chromatin folding determines the accessibility of DNA constituting eukaryotic genomes and consequently is profoundly important in the mechanisms of nuclear processes such as gene regulation. Nucleosome arrays compact to form a 30-nanometer chromatin fiber of hitherto disputed structure. Two competing classes of models have been proposed in which nucleosomes are either arranged linearly in a one-start higher order helix or zigzag back and forth in a two-start helix. We analyzed compacted nucleosome arrays stabilized by introduction of disulfide cross-links and show that the chromatin fiber comprises two stacks of nucleosomes in accord with the two-start model.

引用

页码：1571 / 1573

页数：3