Chemical profiling of Qixue Shuangbu Tincture by ultra-performance liquid chromatography with electrospray ionization quadrupole-time-of-flight high-definition mass spectrometry (UPLC-QTOF/MS)

被引：19

作者：

Chen Lin-Wei ^{[1
,2
]}

Wang Qin ^{[1
]}

Qin Kun-Ming ^{[1
,2
]}

Wang Xiao-Li ^{[1
]}

Wang Bin ^{[1
]}

Chen Dan-Ni ^{[1
]}

Cai Bao-Chang ^{[1
,2
]}

Cai Ting ^{[3
]}

机构：

[1] Nanjing Univ Chinese Med, Coll Pharm, Nanjing 210023, Jiangsu, Peoples R China

[2] Nanjing Haichang Chinese Med Grp Corp, Nanjing 210061, Jiangsu, Peoples R China

[3] China Pharmaceut Univ, Sch Pharm, Nanjing 210009, Peoples R China

来源：

CHINESE JOURNAL OF NATURAL MEDICINES | 2016年 / 14卷 / 02期

关键词：

Qixue Shuangbu Tincture; UPLC-QTOF/MS; Chemical profiling; Traditional Chinese medicine; Q-TOF-MS; CONSTITUENTS; DECOCTION; TANG;

D O I：

10.1016/S1875-5364(16)60007-9

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

The present study was designed to develop and validate a sensitive and reliable ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) method to separate and identify the chemical constituents of Qixue Shuangbu Tincture (QXSBT), a classic traditional Chinese medicine (TCM) prescription. Under the optimized UPLC and QTOF/MS conditions, 56 components in QXSBT, including chalcones, triterpenoids, protopanaxatriol, flavones and flavanones were identified and tentatively characterized within a running time of 42 min. The components were identified by comparing the retention times, accurate mass, and mass spectrometric fragmentation characteristic ions, and matching empirical molecular formula with that of the published compounds. In conclusion, the established UPLC-QTOF/MS method was reliable for a rapid identification of complicated components in the TCM prescriptions.

引用

页码：141 / 146

页数：6

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