Polymerase Chain Reaction-Based Sex Identification in the Greater Roadrunner

被引：7

作者：

Santamaria, Carlos A. ^{[1
]}

Kelley, Samuel ^{[2
]}

Schulz, Gerral G. ^{[2
]}

Ransom, Dean, Jr. ^{[2
]}

Hurtado, Luis A. ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Wildlife & Fisheries Sci, College Stn, TX 77843 USA

[2] Texas AgriLife Res Ctr, Vernon, TX 76384 USA

来源：

JOURNAL OF WILDLIFE MANAGEMENT | 2010年 / 74卷 / 06期

基金：

美国国家科学基金会;

关键词：

birds; Geococcyx californianus; greater roadrunner; polymerase chain reaction (PCR); sex determination; Texas; SEQUENCE VARIATION; BIRDS; CONSERVATION; PROGRAMS;

D O I：

10.2193/2009-248

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

We determined a polymerase chain reaction (PCR)-based method to identify the sex of greater roadrunners (Geococcyx californianus). We found that previously reported primer combinations used in other bird species did not work reliably in roadrunners. However, a novel primer combination (P2-1237L) allowed for consistent and reliable sex identification of roadrunners. Conducting several PCR replicates per sample helped to identify occasional preferential amplification of the Z band that could occur in females (the heterogametic sex; i.e., WZ). Identifying the sex of individuals will allow for examination of sex-associated differences in home range size and habitat use, population demographics, and parenting behavior, as well as for a better understanding of the social system of roadrunners.

引用

页码：1395 / 1399

页数：5

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