Potentiation of estrogen receptor transcriptional activity by breast cancer amplified sequence 2

被引：32

作者：

Qi, C ^{[1
]}

Zhu, YT ^{[1
]}

Chang, J ^{[1
]}

Yeldandi, AV ^{[1
]}

Rao, MS ^{[1
]}

Zhu, YJ ^{[1
]}

机构：

[1] Northwestern Univ, Feinberg Sch Med, Dept Pathol, Chicago, IL 60611 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 328卷 / 02期

关键词：

breast cancer amplified sequence 2; coactivator; estrogen receptor alpha;

D O I：

10.1016/j.bbrc.2004.12.187

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Breast cancer amplified sequence 2 (BCAS2) was initially identified as a gene that was overexpressed and amplified in some breast cancer cell lines. It was later found to be a component of the spliceosome. Here, we identified BCAS2 as an estrogen receptor (ER) alpha interacting protein by yeast two-hybrid screening. In addition to ERalpha, BCAS2 also interacted with ERbeta, TRbeta, PR, and PPARgamma in a ligand-independent way. Transient transfection assays revealed that overexpression of BCAS2 enhanced while inhibition of BCAS2 expression attenuated the estrogen receptor-mediated transcription. BCAS2 potentiated the activation function-2 (AF-2) activity of ERalpha but had no effect on the AF-1 activity. This study suggested that BCAS2 might play an important role in breast cancer development by increasing the estrogen receptor's function. (C) 2005 Elsevier Inc. All rights reserved.

引用

页码：393 / 398

页数：6

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