Establishment and Characterization of the Novel High-Grade Serous Ovarian Cancer Cell Line OVPA8

被引:27
作者
Tudrej, Patrycja [1 ]
Olbryt, Magdalena [1 ]
Zembala-Nozynska, Ewa [2 ]
Kujawa, Katarzyna A. [1 ]
Cortez, Alexander J. [1 ]
Fiszer-Kierzkowska, Anna [3 ]
Piglowski, Wojciech [3 ]
Nikiel, Barbara [2 ]
Glowala-Kosinska, Magdalena [4 ]
Bartkowska-Chrobok, Aleksandra [5 ]
Smagur, Andrzej [4 ]
Fidyk, Wojciech [4 ]
Lisowska, Katarzyna M. [1 ]
机构
[1] Maria Sklodowskaj Curie Inst, Oncol Ctr, Ctr Translat Res & Mol Biol Canc, Gliwice Branch, Ul Wybrzeze Armii Krajowej 15, PL-44101 Gliwice, Poland
[2] Maria Sklodowskaj Curie Inst, Oncol Ctr, Thumor Pathol Dept, Gliwice Branch, Ul Wybrzeze Armii Krajowej 15, PL-44101 Gliwice, Poland
[3] Maria Sklodowskaj Curie Inst, Oncol Ctr, Mol Diagnost Lab, Gliwice Branch, Ul Wybrzeze Armii Krajowej 15, PL-44101 Gliwice, Poland
[4] Maria Sklodowskaj Curie Inst, Oncol Ctr, Dept Bone Marrow Transplantat & Hematol Oncol, Gliwice Branch, Ul Wybrzeze Armii Krajowej 15, PL-44101 Gliwice, Poland
[5] Andrzej Mielecki Independent Publ Hosp, Dept Hematol & Bone Marrow Transplantat, Ul Dabrowskiego 25, PL-40032 Katowice, Poland
关键词
high-grade serous ovarian cancer; cell line; fibroblast growth factor inhibitor CPL304-110-01; BREAST-CANCER; ORIGIN; PAX8; CONTAMINATION; MDA-MB-435; CARCINOMAS; RESISTANCE; MUTATIONS; PATIENT;
D O I
10.3390/ijms19072080
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
High-grade serous ovarian carcinoma (HGSOC) is the most frequent histological type of ovarian cancer and the one with worst prognosis. Unfortunately, the majority of established ovarian cancer cell lines which are used in the research have unclear histological origin and probably do not represent HGSOC. Thus, new and reliable models of HGSOC are needed. Ascitic fluid from a patient with recurrent HGSOC was used to establish a stable cancer cell line. Cells were characterized by cytogenetic karyotyping and short tandem repeat (STR) profiling. New generation sequencing was applied to test for hot-spot mutations in 50 cancer-associated genes and fluorescence in situ hybridization (FISH) analysis was used to check for TP53 status. Cells were analyzed for expression of several marker genes/proteins by reverse-transcription polymerase chain reaction (RT-PCR), fluorescence-activated cell sorting (FACS), and immunocytochemistry (ICC). Functional tests were performed to compare OVPA8 cells with five commercially available and frequently used ovarian cancer cell lines: SKOV3, A2780, OVCAR3, ES2, and OAW42. Our newly-established OVPA8 cell line shows morphologic and genetic features consistent with HGSOC, such as epithelial morphology, multiple chromosomal aberrations, TP53 mutation, BRCA1 mutation, and loss of one copy of BRCA2. The OVPA8 line has a stable STR profile. Cells are positive for EpCAM, CK19, and CD44; they have relatively low plating efficiency/ability to form spheroids, a low migration rate, and intermediate invasiveness in matrigel, as compared to other ovarian cancer lines. OVPA8 is sensitive to paclitaxel and resistant to cisplatin. We also tested two FGFR inhibitors; OVPA8 cells were resistant to AZD4547 (AstraZeneca, London, UK), but sensitive to the new inhibitor CPL304-110-01 (Celon Pharma, Lomianki/Kielpin, Poland). We have established and characterized a novel cell line, OVPA8, which can be a valuable preclinical model for studies on high-grade serous ovarian cancer.
引用
收藏
页数:26
相关论文
共 43 条
[1]  
Anglesio MS, 2013, PLOS ONE, V8, DOI [10.1371/journal.pone.0072162, 10.1371/annotation/ffcaf179-872f-470b-8bb6-f06d8ba6d03a]
[2]  
[Anonymous], P EUR CANC C AMST NE
[3]  
[Anonymous], NM 000546 5 TP53 C 7
[4]  
[Anonymous], CELL LIN IN VITR SCR
[5]   Different TP53 mutants in p53 overexpressed epithelial ovarian carcinoma can be associated both with altered and unaltered glycolytic and apoptotic profiles [J].
Antoun, Stephanie ;
Atallah, David ;
Tahtouh, Roula ;
Alaaeddine, Nada ;
Moubarak, Malak ;
Khaddage, Abir ;
Ayoub, Eliane Nasr ;
Chahine, George ;
Hilal, George .
CANCER CELL INTERNATIONAL, 2018, 18
[6]   Ovarian Cancer Cell Line Panel (OCCP): Clinical Importance of In Vitro Morphological Subtypes [J].
Beaufort, Corine M. ;
Helmijr, Jean C. A. ;
Piskorz, Anna M. ;
Hoogstraat, Marlous ;
Ruigrok-Ritstier, Kirsten ;
Besselink, Nicolle ;
Murtaza, Muhammed ;
van IJcken, Wilfred F. J. ;
Heine, Anouk A. J. ;
Smid, Marcel ;
Koudijs, Marco J. ;
Brenton, James D. ;
Berns, Els M. J. J. ;
Helleman, Jozien .
PLOS ONE, 2014, 9 (09)
[7]   Side Population is Not Necessary or Sufficient for a Cancer Stem Cell Phenotype in Glioblastoma Multiforme [J].
Broadley, Kate W. R. ;
Hunn, Martin K. ;
Farrand, Kathryn J. ;
Price, Kylie M. ;
Grasso, Carole ;
Miller, Rose J. ;
Hermans, Ian F. ;
McConnell, Melanie J. .
STEM CELLS, 2011, 29 (03) :452-461
[8]   Prevalence of the most frequent BRCA1 mutations in Polish population [J].
Brozek, Izabela ;
Cybulska, Celina ;
Ratajska, Magdalena ;
Piatkowska, Magdalena ;
Kluska, Anna ;
Balabas, Aneta ;
Dabrowska, Michalina ;
Nowakowska, Dorota ;
Niwinska, Anna ;
Pamula-Pilat, Jolanta ;
Tecza, Karolina ;
Pekala, Wioletta ;
Rembowska, Jolanta ;
Nowicka, Karina ;
Mosor, Maria ;
Januszkiewicz-Lewandowska, Danuta ;
Rachtan, Jadwiga ;
Grzybowska, Ewa ;
Nowak, Jerzy ;
Steffen, Jan ;
Limon, Janusz .
JOURNAL OF APPLIED GENETICS, 2011, 52 (03) :325-330
[9]   MDA-MB-435 and M14 Cell Lines: Identical but not M14 Melanoma? [J].
Chambers, Ann F. .
CANCER RESEARCH, 2009, 69 (13) :5292-5293
[10]  
Cobb Lauren Patterson, 2015, Gynecol Oncol Res Pract, V2, P1, DOI 10.1186/s40661-015-0008-z