Response of isolated rat descending vasa recta to bradykinin

被引:28
|
作者
Pallone, TL
Silldorff, EP
Cheung, JY
机构
[1] Univ Maryland, Sch Med, Div Nephrol, Baltimore, MD 21201 USA
[2] Penn State Univ, Milton S Hershey Med Ctr, Div Nephrol, Hershey, PA 17033 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 1998年 / 274卷 / 03期
关键词
microcirculation; microperfusion; renal medulla; receptor; calcium;
D O I
10.1152/ajpheart.1998.274.3.H752
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Outer medullary descending vasa recta (OMDVR) were dissected from the outer medullary vascular bundles of young rats, perfused in vitro, and loaded with fura 2 for measurement of intracellular calcium concentration ([Ca2+](i)) by fluorescent ratio imaging. Fluorescent video images revealed that fura 2 selectively loads into endothelial cells but not pericytes. Bradykinin (BK), at concentrations >10(-11) M, elicited an increase in [Ca2+](i) from baseline values in the range from 50 to 100 nM to peak values of 600-800 nM followed by a sustained plateau of 150-250 nM. The vasopressin V-1-receptor agonist [Phe(2),Ile(3),Orn(8)]vasopressin constricted OMDVR but yielded no observable [Ca2+](i) response, a finding that is consistent with an endothelial cell origin for the fura 2 fluorescent signal. The BK [Ca2+](i) response was blocked by the selective BK B-2-receptor antagonists D-Arg-[Hyp(3),Thi(5,8),D-Phe(7)]BK and D-Arg-[Hyp(3),D-Phe(7),Leu(8)]BK but not the B-1 antagonist des-Arg(9)-[Leu(8)]BK. BK vasodilated microperfused OMDVR that had been preconstricted with 10(-8) M angiotensin II. We conclude that the [Ca2+](i) response of OMDVR endothelia can be selectively studied with fura 2, that BK increases endothelial [Ca2+](i) via the B-2 receptor, and that BK can vasodilate descending vasa recta.
引用
收藏
页码:H752 / H759
页数:8
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