Smartphone-assisted point-of-care colorimetric biosensor for the detection of urea via pH-mediated AgNPs growth

被引：68

作者：

Choi, Cheol-Kyun ^{[1
,3
]}

Shaban, Samy M. ^{[1
,2
,4
]}

Moon, Byeong-Seok ^{[1
]}

Pyun, Do-Gi ^{[3
]}

Kim, Dong-Hwan ^{[1
,2
]}

机构：

[1] Sungkyunkwan Univ SKKU, Sch Chem Engn, Suwon 16419, South Korea

[2] Sungkyunkwan Univ SKKU, Biomed Inst Convergence SKKU BICS, Suwon 16419, South Korea

[3] Biomed Polymer R&D Inst T&L Co Ltd, Anseong 17554, South Korea

[4] Egyptian Petr Res Inst, Petrochem Dept, Cairo, Egypt

来源：

ANALYTICA CHIMICA ACTA | 2021年 / 1170卷

基金：

新加坡国家研究基金会;

关键词：

Urea; Urease; Colorimetric; Point-of-care bioassay; Smartphone-assisted biosensor; CHRONIC KIDNEY-DISEASE; SILVER NANOPARTICLES; IMMOBILIZED UREASE; HYDROGEN-PEROXIDE; QUANTUM DOTS; SENSOR; AMINOPHENOL; ENZYME; PROBE; 4-AMINOPHENOL;

D O I：

10.1016/j.aca.2021.338630

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Smartphone-assisted point-of-care (POC) bioassay has brought a giant leap in personal healthcare sys-tem and environmental monitoring advancements. In this study, we developed a rapid and reliable colorimetric urea biosensor assisted by a smartphone. We employed hydrolysis of urea into NH3 by urease, which activates the reduction power of tannic acid, to generate silver nanoparticles for a dramatic colorimetric response. The proposed urea biosensor was validated in a solution to provide high selec-tivity against various interferents in human urine. It had high sensitivity, with a limit of detection as low as 0.0036 mM, and a high reliability of 99% +/- 2.9% via the standard addition method. The urea biosensor was successfully implanted on a paper to facilitate smartphone-assisted POC readout with a limit of detection of 0.58 mM and wide detection range of 500 mM, whereby direct diagnosis of human urine without dilution was realized. Our smartphone-assisted POC colorimetric urea biosensor will pave the way for daily monitoring systems of renal and hepatic dysfunction diseases. (c) 2021 Elsevier B.V. All rights reserved.

引用

页数：10

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