Conserved nucleosome positioning defines replication origins

被引：283
作者：
Eaton, Matthew L. [1 ]
Galani, Kyriaki [2 ]
Kang, Sukhyun [2 ]
Bell, Stephen P. [2 ]
MacAlpine, David M. [1 ]
机构：
[1] Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA
[2] MIT, Howard Hughes Med Inst, Dept Biol, Cambridge, MA 02139 USA
来源：
GENES & DEVELOPMENT | 2010年 / 24卷 / 08期
基金：
美国国家卫生研究院;
关键词：
DNA replication; origin recognition complex (ORC); nucleosome; chromatin; SACCHAROMYCES-CEREVISIAE; DNA-REPLICATION; CHROMATIN-STRUCTURE; MCM PROTEINS; GENOME; ORC; INITIATION; COMPLEX; BINDING; SEQUENCE;
D O I：
10.1101/gad.1913210
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The origin recognition complex (ORC) specifies replication origin location. The Saccharomyces cerevisiae ORC recognizes the ARS (autonomously replicating sequence) consensus sequence (ACS), but only a subset of potential genomic sites are bound, suggesting other chromosomal features influence ORC binding. Using high-throughput sequencing to map ORC binding and nucleosome positioning, we show that yeast origins are characterized by an asymmetric pattern of positioned nucleosomes flanking the ACS. The origin sequences are sufficient to maintain a nucleosome-free origin; however, ORC is required for the precise positioning of nucleosomes flanking the origin. These findings identify local nucleosomes as an important determinant for origin selection and function.
引用
收藏
页码：748 / 753
页数：6
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