Preparation of PCR products for DNA sequencing

被引：10

作者：

Leonard, JT

Grace, MB

Buzard, GS

Mullen, MJ

Barbagallo, CB

机构：

[1] Millipore Corp, Mol Biol Grp, Analyt Prod Div, Danvers, MA 01923 USA

[2] NIMH, Bethesda, MD 20892 USA

[3] NCI, Frederick Canc Res & Dev Ctr, Frederick, MD USA

来源：

BIOTECHNIQUES | 1998年 / 24卷 / 02期

关键词：

D O I：

10.2144/98242pf01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate that routine PCR product analytical agarose gels can also serve as preparative gels for quick DNA template purification before sequencing. The band of interest is excised, placed into a Gel Nebulizer(TM) inside a Micropure(TM) separator and rapidly purified in a single centrifugation step. Gel-purified PCR product, suitable for manual and automated sequencing, is delivered within 10 min.

引用

页码：314 / 317

页数：4

共 5 条

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[J]. NUCLEIC ACIDS RESEARCH, 1994, 22 (20) : 4354 - 4355

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