Cytologic detection of Toxoplasma gondii in the cerebrospinal fluid of a dog and in vitro isolation of a unique mouse-virulent recombinant strain

被引：2

作者：

Borges-Silva, Waleria ^{[1
]}

Rezende-Gondim, Mariana M. ^{[1
]}

Galvao, Gideao S. ^{[1
]}

Rocha, Daniele S. ^{[2
]}

Albuquerque, George R. ^{[2
]}

Gondim, Luis P. ^{[1
]}

机构：

[1] Univ Fed Bahia, Dept Vet Anat Pathol & Clin, Sch Vet Med & Anim Sci, Av Adhemar Barros 500, BR-40170110 Salvador, Bahia, Brazil

[2] Univ Estadual Santa Cruz, Dept Agr & Environm Sci, Campus Soane Nazare Andrade, Ilheus, BA, Brazil

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2021年 / 33卷 / 03期

关键词：

canine; cerebrospinal fluid; genotype; toxoplasmosis; virulence;

D O I：

10.1177/1040638721996685

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Parasites resembling Neospora caninum or Toxoplasma gondii were detected by cytologic examination of cerebrospinal fluid (CSF) from a dog with neurologic disease. The dog became severely ill and was euthanized. Canine tissue homogenates were used for direct parasite isolation in cell culture, bioassay in 2 mouse lineages, and PCR. T. gondii was isolated in monkey kidney cells, and species identity was confirmed by PCR. Inoculated parasites were highly virulent for mice, which developed clinical signs and were euthanized immediately. PCR-RFLP for T. gondii using the cultured isolate (TgDgBA22) was conducted with 12 genetic markers, and a unique recombinant strain was identified. Detection of T. gondii by CSF cytology, although described in humans, had not been reported previously in dogs, to our knowledge, and was crucial for the diagnosis of toxoplasmosis in the examined dog.

引用

页码：591 / 594

页数：4