Immobilized nicotinic receptor stationary phases: going with the flow in high-throughput screening and pharmacological studies

被引：40

作者：

Moaddel, R ^{[1
]}

Wainer, IW ^{[1
]}

机构：

[1] NIA, Gerontol Res Ctr, NIH, Baltimore, MD 21224 USA

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2003年 / 30卷 / 06期

关键词：

nicotinic agonists; nicotinic antagonists; non-competitive inhibitors; affinity chromatography; immobilized receptors;

D O I：

10.1016/S0731-7085(02)00513-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The nicotinic acetylcholine receptor (nAChR) subtypes alpha3beta4-nAChR and alpha4beta2-nAChR have been immobilized and the resulting stationary phases used to determine binding affinities. The alpha3beta4-nAUR column was coupled to a C-18 column and a mixture of 18 compounds was sorted into ligands and non-ligands for the alpha3beta4-nAUR. The results demonstrate that the nAChR stationary phases can be used for on-line high-throughput screening (HTS). Published by Elsevier Science B.V.

引用

页码：1715 / 1724

页数：10

共 22 条

[1] Multidimensional on-line screening for ligands to the α3β4 neuronal nicotinic acetylcholine receptor using an immobilized nicotinic receptor liquid chromatographic stationary phase [J].