Preparative HPLC Chromatographic Approach for the Rapid Isolation of Phytotoxins from the Fungus Curvularia lunata of Spigelia anthelmia Leaves

被引：0

作者：

Silva, Geraldo Humberto ^{[1
]}

Machado, Samisia M. F. ^{[2
]}

Katchborian-Neto, Albert ^{[3
]}

Pfenning, Ludwig H. ^{[4
]}

Conceicao Santos, Mario Ferreira ^{[5
]}

机构：

[1] Univ Fed Vicosa, Inst Exact & Technol Sci, BR-38810000 Rio Parnaiba, MG, Brazil

[2] Univ Fed Sergipe, Dept Chem, BR-49100000 Sao Cristovao, SE, Brazil

[3] Univ Fed Alfenas, Chem Inst, BR-37130001 Alfenas, MG, Brazil

[4] Univ Fed Lavras, Dept Phytopathol, BR-37200000 Lavras, MG, Brazil

[5] Univ Franca, Res Grp Exact & Technol Sci, BR-14404600 Franca, SP, Brazil

来源：

REVISTA VIRTUAL DE QUIMICA | 2022年

关键词：

Phytotoxins; chromatography; preparative HPLC; PATHOGEN;

D O I：

10.21577/1984-6835.20220038

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In the search for natural herbicides from phytopathogenic fungi, the Curvularia lunata fungal strain was isolated from a leaf spot of the Spigelia anthelmia leaves and then grown in a potato dextrose broth to obtain the initial crude extract. An efficient chromatography method was developed for demonstrating the use of reverse phase isocratic transfer of high-performance liquid chromatography (HPLC) analytical to a preparative scale. The rapid fractionation through preparative HPLC isolated two known compounds. radicinol 1 and radicinin 2, reported in the literature as phytotoxic agents. Although, these substances did not show phytotoxic activity against S. anthelmia leaves when tested at a 100 mu g/mL concentration.

引用

页码：819 / 823

页数：5

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