A small volume technique to examine and compare alveolar macrophage phagocytosis of apoptotic cells and non typeable Haemophilus influenzae (NTHi)
被引:18
作者:
论文数: 引用数:
h-index:
机构:
Ween, Miranda
[1
,2
,3
]
Ahern, Jessica
论文数: 0引用数: 0
h-index: 0
机构:
Royal Adelaide Hosp, Hanson Inst, Lung Res, Adelaide, SA 5000, Australia
Royal Adelaide Hosp, Dept Thorac Med, Adelaide, SA 5000, AustraliaRoyal Adelaide Hosp, Hanson Inst, Lung Res, Adelaide, SA 5000, Australia
Ahern, Jessica
[1
,2
]
Carroll, Alexander
论文数: 0引用数: 0
h-index: 0
机构:
Royal Adelaide Hosp, Hanson Inst, Lung Res, Adelaide, SA 5000, Australia
Royal Adelaide Hosp, Dept Thorac Med, Adelaide, SA 5000, AustraliaRoyal Adelaide Hosp, Hanson Inst, Lung Res, Adelaide, SA 5000, Australia
Carroll, Alexander
[1
,2
]
论文数: 引用数:
h-index:
机构:
Hodge, Greg
[1
,2
,3
]
Pizzutto, Susan
论文数: 0引用数: 0
h-index: 0
机构:
Charles Darwin Univ, Menzies Sch Hlth Res, Darwin, NT 0909, AustraliaRoyal Adelaide Hosp, Hanson Inst, Lung Res, Adelaide, SA 5000, Australia
Pizzutto, Susan
[4
]
Jersmann, Hubertus
论文数: 0引用数: 0
h-index: 0
机构:
Royal Adelaide Hosp, Hanson Inst, Lung Res, Adelaide, SA 5000, Australia
Royal Adelaide Hosp, Dept Thorac Med, Adelaide, SA 5000, Australia
Univ Adelaide, Dept Med, Adelaide, SA 5001, AustraliaRoyal Adelaide Hosp, Hanson Inst, Lung Res, Adelaide, SA 5000, Australia
Jersmann, Hubertus
[1
,2
,3
]
论文数: 引用数:
h-index:
机构:
Reynolds, Paul
[1
,2
,3
]
Hodge, Sandra
论文数: 0引用数: 0
h-index: 0
机构:
Royal Adelaide Hosp, Hanson Inst, Lung Res, Adelaide, SA 5000, Australia
Royal Adelaide Hosp, Dept Thorac Med, Adelaide, SA 5000, Australia
Univ Adelaide, Dept Med, Adelaide, SA 5001, AustraliaRoyal Adelaide Hosp, Hanson Inst, Lung Res, Adelaide, SA 5000, Australia
Hodge, Sandra
[1
,2
,3
]
机构:
[1] Royal Adelaide Hosp, Hanson Inst, Lung Res, Adelaide, SA 5000, Australia
[2] Royal Adelaide Hosp, Dept Thorac Med, Adelaide, SA 5000, Australia
[3] Univ Adelaide, Dept Med, Adelaide, SA 5001, Australia
[4] Charles Darwin Univ, Menzies Sch Hlth Res, Darwin, NT 0909, Australia
A defective ability of alveolar macrophages to phagocytose both apoptotic airway epithelial cells and bacteria in chronic lung diseases potentially associated with inflammation and bacterial colonisation of the lower airways, often with non-typeable Haemophilus influenzae (NTHi), has been shown. We routinely assess phagocytosis in the airway of children: however, the small volume of BAL obtained usually precludes the investigation of phagocytosis of both (potentially equally relevant) apoptotic cells and NTHi. Methods: We established a 'one-tube, dual target' flow-cytometric assay for investigating alveolar macrophage phagocytosis of both apoptotic cells and NTHi. The effect of bacterial presence on phagocytosis of apoptotic cells was assessed by comparing results using this technique to standard 'two tube, single target' methods. The comparative ability of alveolar macrophages to phagocytose NTHi or apoptotic cells was assessed in 10/group of healthy adult controls and patients with COPD, 12 children with bronchiectasis, and 10 children controls. We then assessed the influence of increasing concentrations of NTHi targets on the ability of THP-1 macrophages to simultaneously phagocytose apoptotic cells. Results: Alveolar macrophages phagocytosed NTHi more avidly than apoptotic cells (mean +/- SEM: apoptotic cells 15.4% +/- 0.5 vs. NTHi 17.2% +/- 0.7, p < 0.05). The presence of NTHi targets (ratio of 1:100 macrophage: NTHi; 2 x 10(7) CFU routinely applied in our assay) had no effect on the ability of macrophages to simultaneously phagocytose apoptotic cells. However, when bacterial numbers were increased (up to 4-fold) there was a small but significant suppressive effect on the ability of macrophages to phagocytose apoptotic cells. Conclusions: We describe a small volume 'one tube, dual target' technique to measure phagocytosis of apoptotic cells and NTHi. We show that alveolar macrophages phagocytose NTHi more avidly than apoptotic cells, and that an increased presence of NTHi in the airway may reduce the ability of alveolar macrophages to phagocytose apoptotic cells. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:7 / 14
页数:8
相关论文
共 39 条
[1]
[Anonymous], GLOB STRAT DIAGN MAN
[2]
Barnes P.J., 2006, Kendig's Disorders of the Respiratory Tract in Children, P65
机构:
Univ Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Univ Virginia, Dept Pharmacol, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Chekeni, Faraaz B.
Elliott, Michael R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Univ Virginia, Ctr Cell Clearance, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Elliott, Michael R.
Sandilos, Joanna K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Dept Pharmacol, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Sandilos, Joanna K.
Walk, Scott F.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Univ Virginia, Ctr Cell Clearance, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Walk, Scott F.
Kinchen, Jason M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Univ Virginia, Ctr Cell Clearance, Charlottesville, VA 22908 USA
Univ Virginia, Dept Microbiol, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Kinchen, Jason M.
Lazarowski, Eduardo R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ N Carolina, Dept Pharmacol, Chapel Hill, NC 27599 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Lazarowski, Eduardo R.
Armstrong, Allison J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Univ Virginia, Ctr Cell Clearance, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Armstrong, Allison J.
Penuela, Silvia
论文数: 0引用数: 0
h-index: 0
机构:
Univ Western Ontario, Dept Anat & Cell Biol, London, ON N6A 5C1, CanadaUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Penuela, Silvia
Laird, Dale W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Western Ontario, Dept Anat & Cell Biol, London, ON N6A 5C1, CanadaUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Laird, Dale W.
Salvesen, Guy S.
论文数: 0引用数: 0
h-index: 0
机构:
Burnham Inst Med Res, Program Apoptosis & Cell Death Res, La Jolla, CA 92037 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Salvesen, Guy S.
Isakson, Brant E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Cardiovasc Res Ctr, Charlottesville, VA 22908 USA
Univ Virginia, Dept Mol Physiol & Biol Phys, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Isakson, Brant E.
Bayliss, Douglas A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Dept Pharmacol, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Bayliss, Douglas A.
Ravichandran, Kodi S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Univ Virginia, Ctr Cell Clearance, Charlottesville, VA 22908 USA
Univ Virginia, Dept Microbiol, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
机构:
Hadassah Hebrew Univ, Med Ctr, Dept Pediat, Pulm & Cyst Fibrosis Ctr, IL-91240 Jerusalem, IsraelHadassah Hebrew Univ, Med Ctr, Dept Pediat, Pulm & Cyst Fibrosis Ctr, IL-91240 Jerusalem, Israel
Cohen-Cymberknoh, Malena
Kerem, Eitan
论文数: 0引用数: 0
h-index: 0
机构:
Hadassah Hebrew Univ, Med Ctr, Dept Pediat, Pulm & Cyst Fibrosis Ctr, IL-91240 Jerusalem, IsraelHadassah Hebrew Univ, Med Ctr, Dept Pediat, Pulm & Cyst Fibrosis Ctr, IL-91240 Jerusalem, Israel
Kerem, Eitan
Ferkol, Thomas
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Dept Pediat, St Louis, MO 63130 USAHadassah Hebrew Univ, Med Ctr, Dept Pediat, Pulm & Cyst Fibrosis Ctr, IL-91240 Jerusalem, Israel
Ferkol, Thomas
Elizur, Arnon
论文数: 0引用数: 0
h-index: 0
机构:
Tel Aviv Univ, Sch Med, Assaf Harofeh Med Ctr, Dept Pediat,Inst Asthma Allergy & Immunol, Zerifin, IsraelHadassah Hebrew Univ, Med Ctr, Dept Pediat, Pulm & Cyst Fibrosis Ctr, IL-91240 Jerusalem, Israel
机构:
Univ Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Univ Virginia, Dept Pharmacol, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Chekeni, Faraaz B.
Elliott, Michael R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Univ Virginia, Ctr Cell Clearance, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Elliott, Michael R.
Sandilos, Joanna K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Dept Pharmacol, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Sandilos, Joanna K.
Walk, Scott F.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Univ Virginia, Ctr Cell Clearance, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Walk, Scott F.
Kinchen, Jason M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Univ Virginia, Ctr Cell Clearance, Charlottesville, VA 22908 USA
Univ Virginia, Dept Microbiol, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Kinchen, Jason M.
Lazarowski, Eduardo R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ N Carolina, Dept Pharmacol, Chapel Hill, NC 27599 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Lazarowski, Eduardo R.
Armstrong, Allison J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Univ Virginia, Ctr Cell Clearance, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Armstrong, Allison J.
Penuela, Silvia
论文数: 0引用数: 0
h-index: 0
机构:
Univ Western Ontario, Dept Anat & Cell Biol, London, ON N6A 5C1, CanadaUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Penuela, Silvia
Laird, Dale W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Western Ontario, Dept Anat & Cell Biol, London, ON N6A 5C1, CanadaUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Laird, Dale W.
Salvesen, Guy S.
论文数: 0引用数: 0
h-index: 0
机构:
Burnham Inst Med Res, Program Apoptosis & Cell Death Res, La Jolla, CA 92037 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Salvesen, Guy S.
Isakson, Brant E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Cardiovasc Res Ctr, Charlottesville, VA 22908 USA
Univ Virginia, Dept Mol Physiol & Biol Phys, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Isakson, Brant E.
Bayliss, Douglas A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Dept Pharmacol, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Bayliss, Douglas A.
Ravichandran, Kodi S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
Univ Virginia, Ctr Cell Clearance, Charlottesville, VA 22908 USA
Univ Virginia, Dept Microbiol, Charlottesville, VA 22908 USAUniv Virginia, Beirne B Carter Ctr Immunol Res, Charlottesville, VA 22908 USA
机构:
Hadassah Hebrew Univ, Med Ctr, Dept Pediat, Pulm & Cyst Fibrosis Ctr, IL-91240 Jerusalem, IsraelHadassah Hebrew Univ, Med Ctr, Dept Pediat, Pulm & Cyst Fibrosis Ctr, IL-91240 Jerusalem, Israel
Cohen-Cymberknoh, Malena
Kerem, Eitan
论文数: 0引用数: 0
h-index: 0
机构:
Hadassah Hebrew Univ, Med Ctr, Dept Pediat, Pulm & Cyst Fibrosis Ctr, IL-91240 Jerusalem, IsraelHadassah Hebrew Univ, Med Ctr, Dept Pediat, Pulm & Cyst Fibrosis Ctr, IL-91240 Jerusalem, Israel
Kerem, Eitan
Ferkol, Thomas
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Dept Pediat, St Louis, MO 63130 USAHadassah Hebrew Univ, Med Ctr, Dept Pediat, Pulm & Cyst Fibrosis Ctr, IL-91240 Jerusalem, Israel
Ferkol, Thomas
Elizur, Arnon
论文数: 0引用数: 0
h-index: 0
机构:
Tel Aviv Univ, Sch Med, Assaf Harofeh Med Ctr, Dept Pediat,Inst Asthma Allergy & Immunol, Zerifin, IsraelHadassah Hebrew Univ, Med Ctr, Dept Pediat, Pulm & Cyst Fibrosis Ctr, IL-91240 Jerusalem, Israel