Bioanalytical methods for the metalloproteomics study of bovine longissimus thoracis muscle tissue with different grades of meat tenderness in the Nellore breed (Bos indicus)

被引：25

作者：

Baldassini, Welder Angelo ^{[1
]}

Braga, Camila Pereira ^{[2
]}

Loyola Chardulo, Luis Artur ^{[1
]}

Ii Vasconcelos Silva, Josineudson Augusto ^{[1
]}

Malheiros, Jessica Moraes ^{[3
]}

de Albuquerque, Lucia Galvao ^{[3
]}

Fernandes, Talita Tanaka ^{[2
]}

Padilha, Pedro de Magalhaes ^{[2
]}

机构：

[1] Sao Paulo State Univ UNESP, Coll Vet & Anim Sci, Dept Anim Breeding & Nutr, BR-18618970 Sao Paulo, Brazil

[2] Sao Paulo State Univ UNESP, Inst Biosci, BR-18618970 Sao Paulo, Brazil

[3] Sao Paulo State Univ UNESP, Coll Agr & Vet Sci, Dept Anim Sci, BR-18618970 Sao Paulo, Brazil

来源：

FOOD CHEMISTRY | 2015年 / 169卷

基金：

巴西圣保罗研究基金会;

关键词：

2D-PAGE; Beef cattle; Calcium; Mass spectrometry; Zebu genotype; GEL-ELECTROPHORESIS; PROTEOME ANALYSIS; IMAGE-ANALYSIS; PROTEINS; IDENTIFICATION; LIPOLYSIS;

D O I：

10.1016/j.foodchem.2014.07.131

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The work describes a metalloproteomics study of bovine muscle tissue with different grades of meat tenderness from animals of the Nellore breed (Bos indicus) based on protein separation by two-dimensional gel electrophoresis, the identification of calcium ions in protein spots by X-ray fluorescence (SR-XRF) and the characterisation of proteins by electrospray ionisation mass spectrometry. Forty (40) specimens were selected and divided into two experimental groups: animals with tough meat (TO) and animals with tender meat (TE). A third group (P) of Piedmontese breed animals (Bos taurus) was included to serve as a comparative model for the level of meat tenderness. The procedures were efficient and preserved the metal protein structure, enabling calcium detection in protein spots by SR-XRF at a given molecular weight range of 14-97 kDa. Two proteins (pyruvate kinase and albumin) were inferred to be related to the phenotypical differences in animals from the different groups. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：65 / 72

页数：8

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