Multigaps Embedded Nanoassemblies Enhance In Situ Raman Spectroscopy for Intracellular Telomerase Activity Sensing

被引：119
作者：
Xu, Liguang [1 ]
Zhao, Sen [1 ]
Ma, Wei [1 ]
Wu, Xiaoling [1 ]
Li, Si [1 ]
Kuang, Hua [1 ]
Wang, Libing [1 ]
Xu, Chuanlai [1 ]
机构：
[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Int Joint Res Lab Biointerface & Biodetect, Wuxi 214122, Jiangsu, Peoples R China
来源：
ADVANCED FUNCTIONAL MATERIALS | 2016年 / 26卷 / 10期
基金：
中国国家自然科学基金;
关键词：
CANCER BIOMARKER DETECTION; GOLD NANOPARTICLES; SINGLE-MOLECULE; SCATTERING SERS; DNA DETECTION; ASSEMBLIES; CELLS; NANOSTRUCTURES; DELIVERY; AMPLIFICATION;
D O I：
10.1002/adfm.201504587
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The highly sensitive and quantitative biodetection of intracellular telomerase is challenging. A DNA-driven nanoparticle self-assembling pyramid encoding a Raman reporter (Cy5) is reported that detects telomerase in live cells. In the presence of the target, the telomerase primer is extended and the inner DNA chain is replaced, leading to the reduction in the surface-enhanced Raman scattering (SERS) signal and the simultaneous recovery of the fluorescent signal. The SERS signal has a linear range for the detection of telomerase in situ of 1 x 10(-14) to 5 x 10(-11) IU, with a limit of detection of 6.2 x 10(-15) IU. The fluorescent signal is used to confirm the intracellular telomerase activity, demonstrating the efficacy of the designed pyramid probe. This biosensing strategy provides a reliable and ultrasensitive protocol for the quantification of biomarkers in living cells.
引用
收藏
页码：1602 / 1608
页数：7
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