Effects of S100A9 in a rat model of asthma and in isolated tracheal spirals

被引：30

作者：

Yin, Lei-Miao ^{[1
]}

Li, Hai-Yan ^{[1
]}

Zhang, Qing-Hua ^{[2
]}

Xu, Yu-Dong ^{[1
]}

Wang, Yu ^{[1
]}

Jiang, Yong-Liang ^{[1
]}

Wei, Ying ^{[1
]}

Liu, Yan-Yan ^{[1
]}

Yang, Yong-Qing ^{[1
]}

机构：

[1] Shanghai Univ Tradit Chinese Med, Mol Biol Lab, Yueyang Hosp Integrated Tradit Chinese & Western, Shanghai 200030, Peoples R China

[2] Natl Engn Ctr Biochips Shanghai, Shanghai, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 398卷 / 03期

基金：

中国国家自然科学基金;

关键词：

S100A9; Asthma; Rat; Sprague-Dawley; Trachea; GENE-EXPRESSION; SERIAL ANALYSIS; CALCIUM; ACTIVATORS; SECRETION; MECHANISM; MIGRATION; PROTEINS; RECEPTOR; CELLS;

D O I：

10.1016/j.bbrc.2010.06.116

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

S100A9 is a member of the S100 family of proteins that contain two EF-hand calcium-binding motifs. We previously reported that S100A9 was differentially expressed during the early airway response phase of asthma and can be regulated by acupuncture. To understand the possible role of S100A9 in asthma, the effects of the S100A9 were investigated in a rat model of asthma and in isolated tracheal spirals. The pulmonary function and isometric tension were measured after the administration of purified recombinant S100A9. The results of in vivo experiments showed that S100A9 (0.1 mu g/kg) significantly decreased the pulmonary resistance and increased the dynamic compliance. The in vitro experimental results showed that S100A9 (100, 200, 400, or 800 ng/ml, final concentrations) significantly reduced the isometric tension of isolated tracheal spirals. These results suggest that S100A9 elicits dose-dependent anti-asthmatic effects and may provide further insight into the treatment of asthma. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：547 / 552

页数：6

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