Sequencing exons 5 to 8 of the p53 gene by MALDI-TOF mass spectrometry

被引：102

作者：

Fu, DJ ^{[1
]}

Tang, K

Braun, A

Reuter, D

Darnhofer-Demar, B

Little, DP

O'Donnell, MJ

Cantor, CR

Köster, H

机构：

[1] Sequenom Inc, San Diego, CA 92121 USA

[2] Boston Univ, Ctr Adv Biotechnol, Boston, MA 02215 USA

来源：

NATURE BIOTECHNOLOGY | 1998年 / 16卷 / 04期

关键词：

genomics; DNA analysis; gene mutation;

D O I：

10.1038/nbt0498-381

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Matrix-assisted laser desorption ionization time of flight mass spectrometry was used to sequence exons 5 to 8 of the human p53 gene. A single tube procedure was established for target amplification and mass spectrometric (MS) sequencing. The MS sequencing scheme is designed for high throughput and parallel sample processing, and is amenable to full automation. Reliable sequencing data were obtained using fmol sample amounts. The high resolution and accuracy of MS sequencing was demonstrated by direct sequencing of a heterozygous template.

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页码：381 / 384

页数：4

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