A Conserved PMK-1/p38 MAPK Is Required in Caenorhabditis elegans Tissue-specific Immune Response to Yersinia pestis Infection

被引:102
作者
Bolz, Devin D. [1 ]
Tenor, Jennifer L. [1 ]
Aballay, Alejandro [1 ]
机构
[1] Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA
基金
美国国家卫生研究院;
关键词
GATA TRANSCRIPTION FACTOR; C-ELEGANS; INNATE IMMUNITY; VIRULENCE FACTORS; OUTER PROTEINS; EXPRESSION; BACTERIAL; DEFENSE; ANTIGEN; SYSTEM;
D O I
10.1074/jbc.M109.091629
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Yersinia pestis has acquired a variety of complex strategies that enable the bacterium to overcome defenses in different hosts and ensure its survival and successful transmission. A full-genome microarray analysis on Caenorhabditis elegans infected with Y. pestis shows enrichment in genes that are markers of innate immune responses and regulated by a conserved PMK-1/p38 MAPK. Consistent with a role in regulating expression of immune effectors, inhibition of PMK-1/p38 by mutation or RNA interference enhances susceptibility to Y. pestis. Further studies of mosaic animals where PMK-1/p38 is exclusively inhibited or overexpressed in a tissue-specific manner indicate that PMK-1/p38 controls expression of a CUB-like family of immune genes at the cell-autonomous level. Given the conserved nature of PMK-1/p38 MAPK-mediated signaling and innate immune responses, PMK-1/p38 MAPK may play a role in the immune response against Y. pestis in natural hosts.
引用
收藏
页码:10832 / 10840
页数:9
相关论文
共 45 条
[1]   Yersinia pestis, the cause of plague, is a recently emerged clone of Yersinia pseudotuberculosis [J].
Achtman, M ;
Zurth, K ;
Morelli, C ;
Torrea, G ;
Guiyoule, A ;
Carniel, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (24) :14043-14048
[2]   RETRACTED: The structure of complement C3b provides insights into complement activation and regulation (Retracted article. See vol. 532,2016) [J].
Ajees, A. Abdul ;
Gunasekaran, K. ;
Volanakis, John E. ;
Narayana, Sthanam. V. L. ;
Kotwal, Girish J. ;
Krishna Murthy, H. M. .
NATURE, 2006, 444 (7116) :221-225
[3]   BABELOMICS: a suite of web tools for functional annotation and analysis of groups of genes in high-throughput experiments [J].
Al-Shahrour, F ;
Minguez, P ;
Vaquerizas, JM ;
Conde, L ;
Dopazo, J .
NUCLEIC ACIDS RESEARCH, 2005, 33 :W460-W464
[4]   Specificity and complexity of the Caenorhabditis elegans innate immune response [J].
Alper, Scott ;
McBride, Sandra J. ;
Lackford, Brad ;
Freedman, Jonathan H. ;
Schwartz, David A. .
MOLECULAR AND CELLULAR BIOLOGY, 2007, 27 (15) :5544-5553
[5]   Regulation of the Caenorhabditis elegans oxidative stress defense protein SKN-1 by glycogen synthase kinase-3 [J].
An, JH ;
Vranas, K ;
Lucke, M ;
Inoue, H ;
Hisamoto, N ;
Matsumoto, K ;
Blackwell, TK .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (45) :16275-16280
[6]   Intraspecies and temperature-dependent variations in susceptibility of Yersinia pestis to the bactericidal action of serum and to polymyxin B [J].
Anisimov, AP ;
Dentovskaya, SV ;
Titareva, GM ;
Bakhteeva, IV ;
Shaikhutdinova, RZ ;
Balakhonov, SV ;
Lindner, B ;
Kocharova, NA ;
Senchenkova, SN ;
Holst, O ;
Pier, GB ;
Knirel, YA .
INFECTION AND IMMUNITY, 2005, 73 (11) :7324-7331
[7]   Resistance of Yersinia pestis to complement-dependent killing is mediated by the Ail outer membrane protein [J].
Bartra, Sara Schesser ;
Styer, Katie L. ;
O'Bryant, Deanna M. ;
Nilles, Matthew L. ;
Hinnebusch, B. Joseph ;
Aballay, Alejandro ;
Plano, Gregory V. .
INFECTION AND IMMUNITY, 2008, 76 (02) :612-622
[8]  
BOMAN HG, 1981, TRENDS BIOCHEM SCI, V6, P306, DOI 10.1146/annurev.mi.41.100187.000535
[9]   THE CUB DOMAIN - A WIDESPREAD MODULE IN DEVELOPMENTALLY-REGULATED PROTEINS [J].
BORK, P ;
BECKMANN, G .
JOURNAL OF MOLECULAR BIOLOGY, 1993, 231 (02) :539-545
[10]  
BRENNER S, 1974, GENETICS, V77, P71