The interactions of fullerene C60 and Benzo(α)pyrene influence their bioavailability and toxicity to zebrafish embryos

被引：31

作者：

Della Torre, Camilla ^{[1
]}

Maggioni, Daniela ^{[2
]}

Ghilardi, Anna ^{[1
]}

Parolini, Marco ^{[3
]}

Santo, Nadia ^{[1
]}

Landi, Claudia ^{[4
]}

Madaschi, Laura ^{[1
]}

Magni, Stefano ^{[1
]}

Tasselli, Stefano ^{[5
]}

Ascagni, Miriam ^{[1
]}

Bini, Luca ^{[4
]}

La Porta, Caterina ^{[3
]}

Del Giacco, Luca ^{[1
]}

Binelli, Andrea ^{[1
]}

机构：

[1] Univ Milan, Dept Biosci, Milan, Italy

[2] Univ Milan, Dept Chem, Milan, Italy

[3] Univ Milan, Dept Environm Sci & Policy, Milan, Italy

[4] Univ Siena, Dept Life Sci, Siena, Italy

[5] CNR IRSA Natl Res Council, Water Res Inst, Brugherio, Italy

来源：

ENVIRONMENTAL POLLUTION | 2018年 / 241卷

关键词：

Fullerene nanoparticles; Danio rerio; Oxidative stress; Proteomics; Trojan horse effect; DANIO-RERIO EMBRYOS; POLYCYCLIC AROMATIC-HYDROCARBONS; WALLED CARBON NANOTUBES; ACID-BINDING PROTEINS; DIPHOSPHATE KINASE-B; DAPHNIA-MAGNA; AQUEOUS SUSPENSIONS; MASS-SPECTROMETRY; OXIDATIVE STRESS; PROTEOMIC ANALYSIS;

D O I：

10.1016/j.envpol.2018.06.042

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study aimed to assess the toxicological consequences related to the interaction of fullerene nano particles (C-60) and Benzo(alpha)pyrene (B(alpha)P) on zebrafish embryos, which were exposed to C-60 and B(alpha)P alone and to C-60 doped with B(alpha)P. The uptake of pollutants into their tissues and intra-cellular localization were investigated by immunofluorescence and electron microscopy. A set of biomarkers of genotoxicity and oxidative stress, as well as functional proteomics analysis were applied to assess the toxic effects due to C-60 interaction with B(alpha)P. The carrier role of C-60 for B(alpha)P was observed, however adsorption on C-60 did not affect the accumulation and localization of B(alpha)P in the embryos. Instead, C-60 doped with B(alpha)P resulted more prone to sedimentation and less bioavailable for the embryos compared to C-60 alone. As for toxicity, our results suggested that C-60 alone elicited oxidative stress in embryos and a down-regulation of proteins involved in energetic metabolism. The C-60 B(alpha)P induced cellular response mechanisms similar to B(alpha)P alone, but generating greater cellular damages in the exposed embryos. (C) 2018 Elsevier Ltd. All rights reserved.

引用

页码：999 / 1008

页数：10

共 67 条

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