Bacterial contamination of boar semen affects the litter size

被引：107

作者：

Maroto Martin, Luis O. ^{[1
,2
]}

Cruz Munoz, Eduardo ^{[2
]}

De Cupere, Francoise ^{[2
]}

Van Driessche, Edilbert ^{[1
]}

Echemendia-Blanco, Dannele ^{[1
]}

Machado Rodriguez, Jose M. ^{[2
]}

Beeckmans, Sonia ^{[1
]}

机构：

[1] Vrije Univ Brussel, Prot Chem Lab, Inst Mol Biol & Biotechnol, B-1050 Brussels, Belgium

[2] Univ Cent Marta Abreu Villas, Lab Lectinol, Fac Agr & Anim Sci, Santa Clara 54830, Cuba

来源：

ANIMAL REPRODUCTION SCIENCE | 2010年 / 120卷 / 1-4期

关键词：

Boar semen; Escherichia coli; Agglutination; Litter size; ESCHERICHIA-COLI; ANTISPERM ANTIBODIES; SPERM-PREPARATION; HUMAN SPERMATOZOA; FERTILITY; QUALITY;

D O I：

10.1016/j.anireprosci.2010.03.008

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

One hundred and fifteen semen samples were collected from 115 different boars from two farms in Cuba. The boars belonged to five different breeds. Evaluation of the semen sample characteristics (volume, pH, colour, smell, motility of sperm cells) revealed that they meet international standards. The samples were also tested for the presence of agglutinated sperm cells and for bacterial contamination. Seventy five percent of the ejaculates were contaminated with at least one type of bacteria and E. coli was by far the major contaminant, being present in 79% of the contaminated semen samples (n = 68). Other contaminating bacteria belonged to the genera Proteus (n = 31), Serratia (n = 31), Enterobacter (n = 24), Klebsiella (n = 12), Staphylococcus (n = 10), Streptococcus (n = 8) and Pseudomonas (n = 7). Only in one sample anaerobic bacteria were detected. Pearson's analysis of the data revealed that there is a positive correlation between the presence of E. coli and sperm agglutination, and a negative correlation between sperm agglutination and litter size. One-way ANOVA and post hoc Tukey analysis of 378 litters showed that the litter size is significantly reduced when semen is used that is contaminated with spermagglutinating E. coli above a threshold value of 3.5 x 10(3) CFU/ml. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：95 / 104

页数：10

共 54 条

[1] Bacteriospermia in extended porcine semen [J].

Althouse, GC ;

Lu, KG .

THERIOGENOLOGY, 2005, 63 (02) :573-584

[2] Field investigations of bacterial contaminants and their effects on extended porcine semen [J].

Althouse, GC ;

Kuster, CE ;

Clark, SG ;

Weisiger, RM .

THERIOGENOLOGY, 2000, 53 (05) :1167-1176

[3]

ALTHOUSE GC, 1998, P 15 INT PIG VET SOC, V2, P37

[4]

ARAUZ S, 2000, C MERC PROD PORC BUE

[5]

ARREDONDO C, 2001, REV CUB SAL ANIMAL, V23, P73

[6] IS THE SPERM BACTERIAL RATIO A DETERMINING FACTOR IN IMPAIRMENT OF SPERM MOTILITY - AN INVITRO STUDY IN MAN WITH ESCHERICHIA-COLI [J].

AUROUX, MR ;

JACQUES, L ;

MATHIEU, D ;

AUER, J .

INTERNATIONAL JOURNAL OF ANDROLOGY, 1991, 14 (04) :264-270

[7]

BARTOOV B, 1991, ANDROLOGIA, V23, P387

[8] Escherichia coli, fimbriae, bacterial persistence and host response induction in the human urinary tract [J].

Bergsten, G ;

Wullt, B ;

Svanborg, C .

INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 295 (6-7) :487-502

[9] Genes coding for enterotoxins and verotoxins in porcine Escherichia coli strains belonging to different O:K:H serotypes: Relationship with toxic phenotypes [J].

Blanco, M ;

Blanco, JE ;

Gonzalez, EA ;

Mora, A ;

Jansen, W ;

Gomes, TAT ;

Zerbini, LF ;

Yano, T ;

DeCastro, AFP ;

Blanco, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (11) :2958-2963

[10]

Blanco M, 1995, Enferm Infecc Microbiol Clin, V13, P236

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