Fluorescence assay based on the thioflavin T-induced conformation switch of G-quadruplexes for TET1 detection

被引：9

作者：

Chen, Xue ^{[1
,2
,3
]}

Cheng, Ying ^{[1
,2
]}

Wang, Yafen ^{[4
]}

Tang, Jing ^{[1
,2
]}

Wang, Fang ^{[1
,2
,3
]}

Chen, Zilin ^{[1
,2
,3
]}

机构：

[1] Minist Educ, Key Lab Combinatorial Biosynth & Drug Discovery, Hubei Prov Engn & Technol Res Ctr Fluorinated Pha, Wuhan 430071, Peoples R China

[2] Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430071, Peoples R China

[3] Chinese Acad Sci, State Key Lab Of1tansducer Technol, Beijing 100080, Peoples R China

[4] Wuhan Univ, Key Lab Biochem Polymers Minist Educ, Coll Chem & Mol Sci, Hubei Prov Key Lab Allergy & Immunol,Inst Adv Stu, Wuhan 430072, Hubei, Peoples R China

来源：

ANALYST | 2021年 / 146卷 / 07期

基金：

中国国家自然科学基金;

关键词：

ALKALINE-PHOSPHATASE ACTIVITY; DNA; DNAZYMES; ENZYMES; SENSOR;

D O I：

10.1039/d1an00109d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Ten-eleven translocation (TET) dioxygenase is of great significance in cytosine demethylation and in the control of cell differentiation and transformation. Herein, a fluorescence method has been developed for the highly sensitive detection of TET1, a member of the TET dioxygenase family. Based on the ThT-induced specific conformation of the G-quadruplex structure, the fluorescence signal decreased linearly with increasing TET1 concentration. The method shows potential clinical applications in the screening of TET protein inhibitors for anticancer drug discovery.

引用

页码：2126 / 2130

页数：5

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