Whole-brain mapping of monosynaptic inputs to midbrain cholinergic neurons

被引:16
作者
Huerta-Ocampo, Icnelia [1 ]
Dautan, Daniel [1 ,2 ]
Gut, Nadine K. [1 ]
Khan, Bakhtawer [1 ]
Mena-Segovia, Juan [1 ]
机构
[1] Rutgers State Univ, Ctr Mol & Behav Neurosci, Newark, NJ 07103 USA
[2] Ist Italiano Tecnol, Dept Neurosci & Brain Technol, Genet Cognit Lab, Via Morego 30, I-16163 Genoa, Italy
关键词
NUCLEUS TEGMENTI PEDUNCULOPONTINUS; SUBSTANTIA-NIGRA; EFFERENT CONNECTIONS; SUPERIOR COLLICULUS; RAT; INNERVATION; PROJECTIONS; AFFERENT; LATERODORSAL; THALAMUS;
D O I
10.1038/s41598-021-88374-6
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The cholinergic midbrain is involved in a wide range of motor and cognitive processes. Cholinergic neurons of the pedunculopontine (PPN) and laterodorsal tegmental nucleus (LDT) send long-ranging axonal projections that target sensorimotor and limbic areas in the thalamus, the dopaminergic midbrain and the striatal complex following a topographical gradient, where they influence a range of functions including attention, reinforcement learning and action-selection. Nevertheless, a comprehensive examination of the afferents to PPN and LDT cholinergic neurons is still lacking, partly due to the neurochemical heterogeneity of this region. Here we characterize the whole-brain input connectome to cholinergic neurons across distinct functional domains (i.e. PPN vs LDT) using conditional transsynaptic retrograde labeling in ChAT::Cre male and female rats. We reveal that input neurons are widely distributed throughout the brain but segregated into specific functional domains. Motor related areas innervate preferentially the PPN, whereas limbic related areas preferentially innervate the LDT. The quantification of input neurons revealed that both PPN and LDT receive similar substantial inputs from the superior colliculus and the output of the basal ganglia (i.e. substantia nigra pars reticulata). Notably, we found that PPN cholinergic neurons receive preferential inputs from basal ganglia structures, whereas LDT cholinergic neurons receive preferential inputs from limbic cortical areas. Our results provide the first characterization of inputs to PPN and LDT cholinergic neurons and highlight critical differences in the connectome among brain cholinergic systems thus supporting their differential roles in behavior.
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页数:14
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