Assessing effects of flow alteration on macroinvertebrate assemblages in Australian dryland rivers

P>1. Possible impacts of water-resource development on assemblages of freshwater macroinvertebrates were investigated in the upper Darling River and some of its tributaries in north-western New South Wales (Australia), an arid and semi-arid region of low relief where alteration of river flows has intensified through expansion of irrigated agriculture. 2. Study sites were grouped into four hydrological regimes resulting from impoundment, flow regulation, water abstraction and natural variation, namely (i) intermittent flow with relatively little hydrological alteration from water-resource development, (ii) intermittent flow with substantial alteration, (iii) near-perennial flow with substantial alteration but unimpounded and (iv) near-perennial flow with substantial alteration plus impoundment by weirs that stabilise water levels. 3. Macroinvertebrates were sampled with three methods (a quantitative cylinder sampler, handnet sampling and baited traps) in three periods with differing hydrology (recessional low flow in June 2003, high flow in March 2004 and increasing flow after drought in December 2004). 4. Taxonomic richness, assemblage composition and catch per unit effort of the crayfish Cherax destructor differed significantly among the site groups, but total macroinvertebrate density and the AUSRIVAS O/E (Australian River Assessment System observed-over-expected) index did not. The principal spatial differences were between the intermittent and near-perennial rivers, and apparent effects of water-resource development and impoundment were more subtle. Temporal differences in richness, abundance and composition were substantial and appeared to be related mainly to variations in discharge and temperature. 5. Current macroinvertebrate-based methods for assessing the 'condition' or 'health' of Australian dryland rivers are inadequate. Such assessments might be improved with (i) reference data that take adequate account of antecedent hydrological conditions, (ii) consideration of long-term taxonomic richness as well as richness on individual sampling occasions, (iii) evaluation of invertebrate population sizes, (iv) analysis of assemblage data by trait composition and (v) adoption of the genus as the default level of taxonomic resolution.