Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination

被引：53

作者：

Krishna de Guzman, Maria ^{[1
]}

Parween, Sabiha ^{[1
]}

Butardo, Vito M. ^{[1
,5
]}

Mae Alhambra, Crisline ^{[1
]}

Anacleto, Roslen ^{[1
]}

Seiler, Christiane ^{[2
]}

Bird, Anthony R. ^{[3
]}

Chow, Chung-Ping ^{[4
,6
]}

Sreenivasulu, Nese ^{[1
]}

机构：

[1] Int Rice Res Inst, Grain Qual & Nutr Ctr, Plant Breeding Div, Los Banos 4030, Laguna, Philippines

[2] Leibniz Inst Plant Genet & Crop Plant Res IPK, Gatersleben, Germany

[3] CSIRO Hlth & Biosecur, Kintore Ave, Adelaide, SA 5000, Australia

[4] Waters Pacific Pte Ltd, Singapore Sci Pk 2, Singapore 117528, Singapore

[5] Charles Sturt Univ, ARC Ind Transformat Training Ctr Funct Grains FGC, Graham Ctr Agr Innovat, Wagga Wagga, NSW 2650, Australia

[6] Genedata AG, CH-4053 Basel, Switzerland

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

RESISTANT STARCH; IN-VITRO; ENZYMATIC MECHANISM; ENDOSPERM MUTANTS; HIGH-AMYLOSE; INDEX; DIGESTION; DIET; WAXY; IDENTIFICATION;

D O I：

10.1038/s41598-017-06026-0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Rice lines with slower starch digestibility provide opportunities in mitigating the global rise in type II diabetes and related non-communicable diseases. However, screening for low glycemic index (GI) in rice breeding programs is not possible due to time and cost constraints. This study evaluated the feasibility of using in vitro cooked grain amylolysis, starch mobilization patterns during seed germination, and variation in starch structure and composition in the mature seed to differentiate patterns of starch digestibility. Mobilization patterns of total starch, resistant starch, amylose and amylopectin chains, and free sugars during seed germination revealed that the process is analogous to digestion in the human gastrointestinal tract. The combination of these biochemical markers can be used as an alternative measure to predict GI. Additionally, transcriptome analysis of stored mRNA transcripts in high and low GI lines detected differences in starch metabolism and confirmed the importance of seed storage pathways in influencing digestibility. Pathway analyses supported by metabolomics data revealed that resistant starch, cell wall non-starch polysaccharides and flavonoids potentially contribute to slower digestibility. These new insights can guide precision breeding programs to produce low GI rice with acceptable cooking quality to help mitigate the burden of diet-associated lifestyle diseases.

引用

页数：14

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