A novel amperometric biosensor based on artichoke (Cynara scolymus L.) tissue homogenate immobilized in gelatin for hydrogen peroxide detection

被引：0
作者：
Öztürk, G
Ertas, FN
Akyilmaz, E
Dinçkaya, E
Tural, H
机构：
[1] Ege Univ, Fac Sci, Dept Chem, TR-35100 Izmir, Turkey
[2] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Izmir, Turkey
来源：
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2004年 / 32卷 / 04期
关键词：
hydrogen peroxide; biosensor; artichoke (Cynara scolymus L.); tissue homogenate based electrode; amperometry;
D O I：
10.1081/LABB-200039660
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A biosensor for specific determination of hydrogen peroxide was developed by using homogenized artichoke (Cynara scolymus L.) tissue in combination with a dissolved oxygen probe and applied in determination of hydrogen peroxide in milk samples. Artichoke tissue, which has catalase activity, was immobilized with gelatine by means of glutaraldehyde and fixed on a pretreated teflon membrane. The electrode response was maximum when 0.05 M phosphate buffer was used at pH 7.0 and at 30degreesC. Upon addition of hydrogen peroxide, the electrode gives a linear response in a concentration range of 5.0-50x10(-5) M with a response time of 3 min. The method was also applied to the determination of hydrogen peroxide in milk samples.
引用
收藏
页码：637 / 645
页数：9
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