Protective role of diosmin against testosterone propionate-induced prostatic hyperplasia in Wistar rats: Plausible role of oxidative stress and inflammation

被引:20
作者
Vafa, A. [1 ]
Afzal, S. M. [1 ]
Barnwal, P. [1 ]
Rashid, S. [1 ,2 ]
Shahid, A. [1 ]
Alpashree [1 ]
Islam, J. [1 ]
Sultana, S. [1 ]
机构
[1] Jamia Hamdard, Dept Med Elementol & Toxicol, Sect Mol Carcinogenesis & Chemoprevent, Sch Chem & Life Sci, New Delhi 110062, India
[2] Prince Sattam Bin Abdulaziz Univ, Dept Pharmacol & Toxicol, Al Kharj, Saudi Arabia
关键词
Diosmin; testosterone propionate; prostatic hyperplasia; ROS; inflammation; NF-KAPPA-B; URINARY-TRACT SYMPTOMS; GLUTATHIONE; CANCER; INJURY; CELLS; 5-ALPHA-REDUCTASE; AMELIORATION; FLAVONOIDS; ANDROGENS;
D O I
10.1177/0960327119889655
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Benign prostatic hyperplasia (BPH) is an important key health concern for aging men. Polyphenolic compounds have been found to possess important roles in the inhibition of numerous ailments that involve reactive oxygen species and inflammation. Diosmin is a citrus flavone that possesses antioxidant, anti-inflammatory, antiproliferative, and anticancer activities, so based on these properties of diosmin, we decided to evaluate its effect on testosterone propionate (TP)-induced BPH. A total of 30 Wistar rats were randomly assigned to five groups having six animals in each. This study was of 28 days in which TP (5 mg kg(-1)) was administered to induce BPH in the last 10 days of the study. It was found that diosmin at the doses of 20 and 40 mg kg(-1) significantly reduced malondialdehyde and xanthine oxidase formation in a dose-dependent manner; however, it replenished catalase, glutathione (GSH), and GSH-dependent enzymes, that is, glutathione peroxidase, glutathione reductase, and glutathione-S-transferase significantly against TP-induced BPH. Further, immunohistochemical study showed that diosmin alleviated inflammatory markers (nuclear factor kappa-light-chain-enhancer of activated B cells, cyclooxygenase-2, and interleukin-6). It was also found that diosmin downregulated the expression of androgen receptor and decreased the prostate-specific antigen concentration dose-dependently, significantly against TP-induced BPH. Diosmin also restored histoarchitecture of the prostate in a dose-dependent manner. Findings from the present study revealed the protective role of diosmin against TP-induced BPH in Wistar rats.
引用
收藏
页码:1133 / 1146
页数:14
相关论文
共 62 条
  • [11] NF-kappa B: Ten years after
    Baeuerle, PA
    Baltimore, D
    [J]. CELL, 1996, 87 (01) : 13 - 20
  • [12] Benzo(a)pyrene induces lung toxicity and inflammation in mice: prevention by carvacrol
    Barnwal, P.
    Vafa, A.
    Afzal, S. M.
    Shahid, A.
    Hasan, S. K.
    Alpashree
    Sultana, S.
    [J]. HUMAN & EXPERIMENTAL TOXICOLOGY, 2018, 37 (07) : 752 - 761
  • [13] CARLBERG I, 1975, J BIOL CHEM, V250, P5475
  • [14] Inhibitory Effect of Diosgenin on Experimentally Induced Benign Prostatic Hyperplasia in Rats
    Chen, Jing
    Zhang, Huai-fen
    Xiong, Chao-mei
    Ruan, Jin-lan
    [J]. JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES, 2016, 36 (06) : 806 - 810
  • [15] Claiborne A., 1985, CRC handbook of methods for oxygen radical research, V1, P283
  • [16] Benign Prostatic Hyperplasia: A New Metabolic Disease of the Aging Male and Its Correlation with Sexual Dysfunctions
    Corona, Giovanni
    Vignozzi, Linda
    Rastrelli, Giulia
    Lotti, Francesco
    Cipriani, Sarah
    Maggi, Mario
    [J]. INTERNATIONAL JOURNAL OF ENDOCRINOLOGY, 2014, 2014
  • [17] Evaluation of the therapeutic effect against benign prostatic hyperplasia and the active constituents from Epilobium angustifolium L.
    Deng, Liqing
    Zong, Wei
    Tao, Xueying
    Liu, Shuang
    Feng, Zhiying
    Lin, Yuping
    Liao, Zhihua
    Chen, Min
    [J]. JOURNAL OF ETHNOPHARMACOLOGY, 2019, 232 : 1 - 10
  • [18] Diet, androgens, oxidative stress and prostate cancer susceptibility
    Fleshner, NE
    Klotz, LH
    [J]. CANCER AND METASTASIS REVIEWS, 1998, 17 (04) : 325 - 330
  • [19] Androgens and prostate cancer: Biology, pathology and hormonal therapy
    Galbraith, SM
    Duchesne, GM
    [J]. EUROPEAN JOURNAL OF CANCER, 1997, 33 (04) : 545 - 554
  • [20] Promising Molecular Targets and Biomarkers for Male BPH and LUTS
    Gharaee-Kermani, Mehrnaz
    Macoska, Jill A.
    [J]. CURRENT UROLOGY REPORTS, 2013, 14 (06) : 628 - 637