miR-21 is negatively correlated with miR-203 in esophageal cancer tissues and functions oppositely in tumor progression

被引:0
作者
Li, Chenwei [1 ]
Li, Kaimin [1 ]
Wang, Zishan [1 ]
Guo, Jing [1 ]
Li, Xinjian [1 ]
机构
[1] Ningbo First Hosp, Dept Thorac Surg, 59 Liuting Rd, Ningbo 315010, Zhejiang, Peoples R China
来源
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE | 2017年 / 10卷 / 07期
关键词
Esophageal cancer; miR-21; miR-203; cell proliferation; negative correlation; MICRORNA; PATHOGENESIS; EXPRESSION; PROGNOSIS; SURVIVAL;
D O I
暂无
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Objectives: It is well-known that microRNAs (miRNAs) function as vital regulators in the development of human malignancies as tumor suppressors or oncogenes. MiR-21 and miR-203 have been both characterized as functional miRNAs in esophageal cancer. This study aimed to evaluate the internal relationship between the two miRNAs. Methods: A total of 56 patients of esophageal cancer were enrolled and expression of miR-21 and miR203 were detected by qRT-PCR. Associations between the two miRNAs expression in different categories of these esophageal cancer samples were analyzed. Inhibitor of miR-21 and/or mimics of miR-203 was introduced into EC9706 cells and cell proliferation was measured by crystal violet staining assay. Results: MiR-21 expression was significantly elevated while miR-203 was decreased in tissue samples of esophageal cancers compared to those in the paired normal adjacent tissues. Negative correlation was found in these two miRNAs expression. Crystal violet staining assays validated that inhibition of miR-21 or forced expression of miR-203 promoted cell proliferation. In addition, inhibition of miR-21 expression led to obvious upregulation of miR-203, and forced miR-203 expression caused miR-21 repression. Conclusion: Negative correlation and opposite functions of miR-21 and miR-203 were established in esophageal cancer. The future combined assay of miR-21 and miR-203 may help to treat this disease.
引用
收藏
页码:10724 / 10729
页数:6
相关论文
共 50 条
  • [31] Relative expressions of miR-205-5p, miR-205-3p, and miR-21 in tissues and serum of non-small cell lung cancer patients
    Jiang, Min
    Zhang, Peng
    Hu, Guozhu
    Xiao, Zuke
    Xu, Fanghua
    Zhong, Ting
    Huang, Fang
    Kuang, Haibin
    Zhang, Wei
    MOLECULAR AND CELLULAR BIOCHEMISTRY, 2013, 383 (1-2) : 67 - 75
  • [32] miR-203 regulates the proliferation, apoptosis and cell cycle progression of pancreatic cancer cells by targeting Survivin
    Xu, Dong
    Wang, Qingguang
    An, Yong
    Xu, Lijian
    MOLECULAR MEDICINE REPORTS, 2013, 8 (02) : 379 - 384
  • [33] Influence of exosome-derived miR-21 on chemotherapy resistance of esophageal cancer
    Yang, Y-C
    Liu, G-J
    Yuan, D-F
    Li, C-Q
    Xue, M.
    Chen, L-J
    EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2019, 23 (04) : 1513 - 1519
  • [34] High expression of serum miR-21 and tumor miR-200c associated with poor prognosis in patients with lung cancer
    Xiao-Guang Liu
    Wang-Yu Zhu
    Yan-Yan Huang
    Li-Na Ma
    Shi-Quan Zhou
    Ye-Kai Wang
    Fang Zeng
    Ji-Hang Zhou
    Yong-Kui Zhang
    Medical Oncology, 2012, 29 : 618 - 626
  • [35] Association of miR-203 Expression with Prognostic Value in Patients with Esophageal Cancer: A Systematic Review and Meta-Analysis
    Cheng, Qirun
    Chen, Lipeng
    Ni, Liping
    JOURNAL OF INVESTIGATIVE SURGERY, 2023, 36 (01)
  • [36] High expression of serum miR-21 and tumor miR-200c associated with poor prognosis in patients with lung cancer
    Liu, Xiao-Guang
    Zhu, Wang-Yu
    Huang, Yan-Yan
    Ma, Li-Na
    Zhou, Shi-Quan
    Wang, Ye-Kai
    Zeng, Fang
    Zhou, Ji-Hang
    Zhang, Yong-Kui
    MEDICAL ONCOLOGY, 2012, 29 (02) : 618 - 626
  • [37] Tumor Expression of miR-10b, miR-21, miR-143 and miR-145 Is Related to Clinicopathological Features of Gastric Cancer in a Central European Population
    Obermannova, Radka
    Redova-Lojova, Martina
    Vychytilova-Faltejskova, Petra
    Grell, Peter
    Cho, William C.
    Sachlova, Milana
    Svoboda, Marek
    Vyzula, Rostislav
    Slaby, Ondrej
    ANTICANCER RESEARCH, 2018, 38 (06) : 3719 - 3724
  • [38] CircRNA-0008717 promotes cell proliferation, migration, and invasion by regulating miR-203/Slug in esophageal cancer cells
    Wang, Tao
    Wang, Jian
    Ren, Wei
    Chen, Shuai
    Cheng, Yu-Feng
    Zhang, Xiao-Mei
    ANNALS OF TRANSLATIONAL MEDICINE, 2020, 8 (16)
  • [39] miR-203 inhibits tumor cell migration and invasion via caveolin-1 in pancreatic cancer cells
    Miao, Lifeng
    Xiong, Xianze
    Lin, Yixin
    Cheng, Yao
    Lu, Jiong
    Zhang, Jie
    Cheng, Nansheng
    ONCOLOGY LETTERS, 2014, 7 (03) : 658 - 662
  • [40] Targeting miR-21 with Sophocarpine Inhibits Tumor Progression and Reverses Epithelial-Mesenchymal Transition in Head and Neck Cancer
    Liu, Wei
    Zhang, Beilei
    Chen, Guo
    Wu, Wenjiao
    Zhou, Lin
    Shi, Yaru
    Zeng, Qi
    Li, Yanqiu
    Sun, Youwei
    Deng, Xingming
    Wang, Fu
    MOLECULAR THERAPY, 2017, 25 (09) : 2129 - 2139