Photothermal-triggered system for oligonucleotides delivery from cationic gold nanorods surface: A molecular dynamic investigation

被引:10
作者
Petralia, Salvatore [1 ,2 ]
Forte, Giuseppe [1 ]
Aiello, Morena [1 ]
Nocito, Giuseppe [3 ]
Conoci, Sabrina [3 ]
机构
[1] Univ Catania, Dept Sci Farmaco & Salute, Viale Andrea Doria 6, I-95125 Catania, Italy
[2] STMicroelectronics Srl, Stradale Primosole 50, I-95121 Catania, Italy
[3] Univ Messina, Dept Sci Chim Biol Farmaceut & Ambientali, Viale Ferdinando Stagno dAlcontres 31, I-98168 Messina, Italy
关键词
Photothermal effect; DNA delivery; Gold nanorods; Molecular dynamic;
D O I
10.1016/j.colsurfb.2021.111654
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Bioconjugate gold-based nanostructures combining the plasmonic photothermal effect with photothermaltriggered DNA delivery are appealing materials for medical diagnostic and therapy for cell-based disease. In this study, we demonstrate the use of surface hybridization to prepare DNA-modified gold nanorods to be used as photo-delivery system for single stranded oligonucleotides. The as prepared DNA modified gold nanorods have strong absorption bands in the visible and near-infrared regions in which the absorbed light through photothermal effect, induces a surface temperature increasing up to the melting temperature with consequent DNA release. No evident DNA release was observed below the melting temperature. The experimental data were supported by molecular dynamic simulation investigation, showing the kinetics aspect of dsDNA de-hybridization at gold nanorods surface at temperature below (298 K) and above (333 K) the melting temperature of sequence investigated. We demonstrate that the cationic charges of surfactant, localized at nanorods surface, induce a remarkable de-hybridization of strands DNA, as confirmed by an increasing of hybridization enthalpy value of about 7 kcal/mol and by a faster de-hybridization process, respect the model of gold nanorods without positive charges. These data were corroborated by the increasing of the root mean square deviation value (about 4.4 ?, calculated at 333 K) indicating that the presence of cationic headgroup at gold surface induce separation of the double strand. This finding data paving the way for the development of nanostructured material for photothermal-triggered delivery systems of DNA for gene therapy application.
引用
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页数:8
相关论文
共 31 条
[1]   Gromacs: High performance molecular simulations through multi-level parallelism from laptops to supercomputers [J].
Abraham, Mark James ;
Murtola, Teemu ;
Schulz, Roland ;
Páll, Szilárd ;
Smith, Jeremy C. ;
Hess, Berk ;
Lindah, Erik .
SoftwareX, 2015, 1-2 :19-25
[2]   Light-induced release of DNA from plasmon-resonant nanoparticles: Towards light-controlled gene therapy [J].
Barhoumi, Aoune ;
Huschka, Ryan ;
Bardhan, Rizia ;
Knight, Mark W. ;
Halas, Naomi J. .
CHEMICAL PHYSICS LETTERS, 2009, 482 (4-6) :171-179
[3]   Quantifying the photothermal conversion efficiency of plasmonic nanoparticles by means of terahertz radiation [J].
Breitenborn, H. ;
Dong, J. ;
Piccoli, R. ;
Bruhacs, A. ;
Besteiro, L. V. ;
Skripka, A. ;
Wang, Z. M. ;
Govorov, A. O. ;
Razzari, L. ;
Vetrone, F. ;
Naccache, R. ;
Morandotti, R. .
APL PHOTONICS, 2019, 4 (12)
[4]   The plasmonic engineering of metal nanoparticles for enhanced fluorescence and Raman scattering [J].
Cade, N. I. ;
Ritman-Meer, T. ;
Kwakwa, K. A. ;
Richards, D. .
NANOTECHNOLOGY, 2009, 20 (28)
[5]   DNA-gold nanorod conjugates for remote control of localized gene expression by near infrared irradiation [J].
Chen, CC ;
Lin, YP ;
Wang, CW ;
Tzeng, HC ;
Wu, CH ;
Chen, YC ;
Chen, CP ;
Chen, LC ;
Wu, YC .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (11) :3709-3715
[6]  
Conoci S., 2020, SENSORS MICROSYSTEMS, V629, P186
[7]   Tracking Gold Nanorods' Interaction with Large 3D Pancreatic-Stromal Tumor Spheroids by Multimodal Imaging: Fluorescence, Photoacoustic, and Photothermal Microscopies [J].
Darrigues, Emilie ;
Nima, Zeid A. ;
Nedosekin, Dmitry A. ;
Watanabe, Fumiya ;
Alghazali, Karrer M. ;
Zharov, Vladimir P. ;
Biris, Alexandru S. .
SCIENTIFIC REPORTS, 2020, 10 (01) :3362
[8]   Secondary Structure-Dependent Physicochemical Interaction of Oligonucleotides with Gold Nanorod and Photothermal Effect for Future Applications: A New Insight [J].
Das, Upasana ;
Sahoo, Aditi ;
Haldar, Subhash ;
Bhattacharya, Sudin ;
Mandal, Syam Sundar ;
Gmeiner, William H. ;
Ghosh, Supratim .
ACS OMEGA, 2018, 3 (10) :14349-14360
[9]   DNA-binding by functionalized gold nanoparticles: Mechanism and structural requirements [J].
Goodman, CM ;
Chari, NS ;
Han, G ;
Hong, R ;
Ghosh, P ;
Rotello, VM .
CHEMICAL BIOLOGY & DRUG DESIGN, 2006, 67 (04) :297-304
[10]   Cancer-targeted photothermal therapy using aptamer-conjugated gold nanoparticles [J].
Hong, Eun Ji ;
Kim, Yoon-Seok ;
Choi, Dae Gun ;
Shim, Min Suk .
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, 2018, 67 :429-436