Multiplexed Five-Color Molecular Imaging of Cancer Cells and Tumor Tissues with Carbon Nanotube Raman Tags in the Near-Infrared

被引：115

作者：

Liu, Zhuang ^{[2
]}

Tabakman, Scott ^{[1
]}

Sherlock, Sarah ^{[1
]}

Li, Xiaolin ^{[1
]}

Chen, Zhuo ^{[1
]}

Jiang, Kaili ^{[3
,4
]}

Fan, Shoushan ^{[3
,4
]}

Dai, Hongjie ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[2] Soochow Univ, Jiangsu Key Lab Carbon Based Funct Mat & Devices, Funct Nano & Soft Mat Lab, Suzhou 215123, Peoples R China

[3] Tsinghua Univ, Dept Phys, Beijing 100084, Peoples R China

[4] Tsinghua Univ, Tsinghua Foxconn Nanotechnol Res Ctr, Beijing 100084, Peoples R China

来源：

NANO RESEARCH | 2010年 / 3卷 / 03期

关键词：

Carbon nanotubes; Raman scattering; biomedicine; multiplexed imaging; QUANTUM DOTS; LIVING SUBJECTS; LIVE CELLS; FLUORESCENCE; DNA; NANOPARTICLES; SPECTROSCOPY; SCATTERING; BIOLOGY; EGFR;

D O I：

10.1007/s12274-010-1025-1

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Single-walled carbon nanotubes (SWNTs) with five different C13/C12 isotope compositions and well-separated Raman peaks have been synthesized and conjugated to five targeting ligands in order to impart molecular specificity. Multiplexed Raman imaging of live cells has been carried out by highly specific staining of cells with a five-color mixture of SWNTs. Ex vivo multiplexed Raman imaging of tumor samples uncovers a surprising up-regulation of epidermal growth factor receptor (EGFR) on LS174T colon cancer cells from cell culture to in vivo tumor growth. This is the first time five-color multiplexed molecular imaging has been performed in the near-infrared (NIR) region under a single laser excitation. Near zero interfering background of imaging is achieved due to the sharp Raman peaks unique to nanotubes over the low, smooth autofluorescence background of biological species.

引用

页码：222 / 233

页数：12

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