Genome-wide Dissection of Co-selected UV-B Responsive Pathways in the UV-B Adaptation of Qingke

被引：131

作者：

Zeng, Xingquan ^{[1
,2
,3
]}

Yuan, Hongjun ^{[1
,2
,3
]}

Dong, Xuekui ^{[4
,5
,6
]}

Peng, Meng ^{[5
,6
,7
]}

Jing, Xinyu ^{[5
,6
]}

Xu, Qijun ^{[1
,2
,3
]}

Tang, Tang ^{[4
]}

Wang, Yulin ^{[1
,2
,3
]}

Zha, Sang ^{[1
,2
,3
]}

Gao, Meng ^{[4
]}

Li, Congzhi ^{[4
]}

Shu, Chujin ^{[4
]}

Wei, Zexiu ^{[1
,3
,8
]}

Qimei, Wangmu ^{[1
,2
,3
]}

Basang, Yuzhen ^{[1
,2
,3
]}

Dunzhu, Jiabu ^{[1
,2
,3
]}

Li, Zeqing ^{[9
]}

Bai, Lijun ^{[10
]}

Shi, Jian ^{[4
]}

Zheng, Zhigang ^{[5
,6
]}

Yu, Sibin ^{[5
,6
]}

Fernie, Alisdair R. ^{[11
]}

Luo, Jie ^{[5
,6
,12
]}

Nyima, Tashi ^{[1
,3
]}

机构：

[1] State Key Lab Hulless Barley & Yak Germplasm Reso, Lhasa 850002, Peoples R China

[2] Tibet Acad Agr & Anim Husb Sci, Inst Agr Res, Lhasa 850002, Tibet, Peoples R China

[3] Tibet Acad Agr & Anim Husb Sci, Lhasa 850002, Tibet, Peoples R China

[4] Wuhan Metware Biotechnol Co Ltd, Wuhan, Hubei, Peoples R China

[5] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

[6] Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Wuhan 430070, Peoples R China

[7] Univ Ghent, VIB UGent Ctr Plant Syst Biol, Technol Pk Zwijnaarde 71, B-9052 Ghent, Belgium

[8] Tibet Acad Agr & Anim Husb Sci, Agr Resources & Environm Res Inst, Lhasa 850002, Tibet, Peoples R China

[9] Wuhan Igenebook Biotechnol Co Ltd, Wuhan, Peoples R China

[10] Chengdu Life Baseline Technol Co Ltd, Chengdu 610041, Peoples R China

[11] Max Planck Inst Mol Plant Physiol, D-144776 Potsdam, Germany

[12] Hainan Univ, Coll Trop Crops, Haikou 572208, Hainan, Peoples R China

来源：

MOLECULAR PLANT | 2020年 / 13卷 / 01期

关键词：

Tibetan hulless barley; UV-B radiation; constitutive and induced accumulation; phenylpropanoid; MYB transcription factor; NATURAL VARIATION; PHENYLPROPANOID METABOLISM; FUNCTIONAL-ANALYSIS; ARABIDOPSIS; BARLEY; GENE; EXPRESSION; TYRAMINE; IDENTIFICATION; FLAVONOIDS;

D O I：

10.1016/j.molp.2019.10.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Qingke (Tibetan hulless barley) has long been cultivated and exposed to long-term and strong UV-B radiation on the Tibetan Plateau, which renders it an ideal species for elucidating novel UV-B responsive mechanisms in plants. Here we report a comprehensive metabolite profiling and metabolite-based genome-wide association study (mGWAS) using 196 diverse qingke and barley accessions. Our results demonstrated both constitutive and induced accumulation, and common genetic regulation, of metabolites from different branches of the phenylpropanoid pathway that are involved in UV-B protection. A total of 90 significant mGWAS loci for these metabolites were identified in barley-qingke differentiation regions, and a number of high-level metabolite trait alleles were found to be significantly enriched in qingke, suggesting co-selection of various phenylpropanoids. Upon dissecting the entire phenylpropanoid pathway, we identified some key determinants controlling natural variation of phenylpropanoid content, including three novel proteins, a flavone C-pentosyltransferase, a tyramine hydroxycinnamoyl acyltransferase, and a MYB transcription factor. Our study, furthermore, demonstrated co-selection of both constitutive and induced phenylpropanoids for UV-B protection in qingke.

引用

页码：112 / 127

页数：16

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