Single-layer graphene modulates neuronal communication and augments membrane ion currents

被引：124

作者：

Pampaloni, Niccolo Paolo ^{[1
]}

Lottner, Martin ^{[2
,3
]}

Giugliano, Michele ^{[4
,5
,6
]}

Matruglio, Alessia ^{[7
,15
]}

D'Amico, Francesco ^{[8
]}

Prato, Maurizio ^{[9
,10
,11
]}

Garrido, Jose Antonio ^{[12
,13
,14
]}

Ballerini, Laura ^{[1
]}

Scaini, Denis ^{[1
,8
]}

机构：

[1] Int Sch Adv Studies SISSA, Trieste, Italy

[2] Tech Univ Munich, Walter Schottky Inst, Garching, Germany

[3] Tech Univ Munich, Phys Dept, Garching, Germany

[4] Univ Antwerp, Dept Biomed Sci, Mol Cellular & Network Excitabil, Antwerp, Belgium

[5] Univ Sheffield, Dept Comp Sci, Sheffield, S Yorkshire, England

[6] Ecole Polytech Fed Lausanne, Brain Mind Inst, Lab Neural Microcircuitry, Lausanne, Switzerland

[7] CNR, IOM, Trieste, Italy

[8] Elettra Sincrotrone Trieste SCpA, Trieste, Italy

[9] Univ Trieste, Dept Chem & Pharmaceut Sci, Trieste, Italy

[10] CIC biomaGUNE, Nanobiotechnol Lab, San Sebastian, Spain

[11] Basque Fdn Sci, Ikerbasque, Bilbao, Spain

[12] Catalan Inst Nanosci & Nanotechnol ICN2, Campus UAB, Bellaterra, Spain

[13] Barcelona Inst Sci & Technol, Campus UAB, Bellaterra, Spain

[14] ICREA3, Barcelona, Spain

[15] CERIC ERIC Cent European Res Infrastruct Consorti, Trieste, Italy

来源：

NATURE NANOTECHNOLOGY | 2018年 / 13卷 / 08期

关键词：

CARBON NANOTUBES; SALT-SOLUTIONS; RAT; CHANNELS; EXCITATION; MICROSCOPY; MECHANISM; GRAPHITE; SURFACE;

D O I：

10.1038/s41565-018-0163-6

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The use of graphene-based materials to engineer sophisticated biosensing interfaces that can adapt to the central nervous system requires a detailed understanding of how such materials behave in a biological context. Graphene's peculiar properties can cause various cellular changes, but the underlying mechanisms remain unclear. Here, we show that single-layer graphene increases neuronal firing by altering membrane-associated functions in cultured cells. Graphene tunes the distribution of extracellular ions at the interface with neurons, a key regulator of neuronal excitability. The resulting biophysical changes in the membrane include stronger potassium ion currents, with a shift in the fraction of neuronal firing phenotypes from adapting to tonically firing. By using experimental and theoretical approaches, we hypothesize that the graphene-ion interactions that are maximized when single-layer graphene is deposited on electrically insulating substrates are crucial to these effects.

引用

页码：755 / +

页数：12

共 64 条

[1] Mechanism of Ba2+ block of a mouse inwardly rectifying K+ channel:: differential contribution by two discrete residues [J].