Live-Cell Imaging of Mammalian RNAs with Spinach2

被引：23

作者：

Strack, Rita L. ^{[1
]}

Jaffrey, Samie R. ^{[1
]}

机构：

[1] Cornell Univ, Dept Pharmacol, Weill Med Coll, New York, NY 10021 USA

来源：

RIBOSWITCHES AS TARGETS AND TOOLS | 2015年 / 550卷

关键词：

GREEN-FLUORESCENT PROTEIN; LIVING CELLS; MESSENGER-RNA; FRAGILE-X; DYNAMICS; SEQUESTRATION; VISUALIZATION; APTAMERS; REPEAT; PROBES;

D O I：

10.1016/bs.mie.2014.10.044

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability to monitor RNAs of interest in living cells is crucial to understanding the function, dynamics, and regulation of this important class of molecules. In recent years, numerous strategies have been developed with the goal of imaging individual RNAs of interest in living cells, each with their own advantages and limitations. This chapter provides an overview of current methods of live-cell RNA imaging, including a detailed discussion of genetically encoded strategies for labeling RNAs in mammalian cells. This chapter then focuses on the development and use of "RNA mimics of GFP" or Spinach technology for tagging mammalian RNAs and includes a detailed protocol for imaging 5S and CGG(60) RNA with the recently described Spinach2 tag.

引用

页码：129 / 146

页数：18

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