Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line

被引：11

作者：

Haridas, Parvathi ^{[1
,2
]}

McGovern, Jacqui A. ^{[1
]}

Kashyap, Abhishek S. ^{[1
]}

McElwain, D. L. Sean ^{[1
,2
]}

Simpson, Matthew J. ^{[1
,2
]}

机构：

[1] Queensland Univ Technol, Inst Hlth & Biomed Innovat, Kelvin Grove 4059, Australia

[2] QUT, Sch Math Sci, POB 2434, Brisbane, Qld 4001, Australia

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

澳大利亚研究理事会;

关键词：

MELANOCYTES; EXPRESSION;

D O I：

10.1038/srep24569

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Reliable identification of different melanoma cell lines is important for many aspects of melanoma research. Common markers used to identify melanoma cell lines include: S100; HMB-45; and Melan-A. We explore the expression of these three markers in four different melanoma cell lines: WM35; WM793; SK-MEL-28; and MM127. The expression of these markers is examined at both the mRNA and protein level. Our results show that the metastatic cell line, MM127, cannot be detected using any of the commonly used melanoma-associated markers. This implies that it would be very difficult to identify this particular cell line in a heterogeneous sample, and as a result this cell line should be used with care.

引用

页数：6

共 23 条

[1] The Ludwig Institute for Cancer Research Melbourne Melanoma Cell Line Panel [J].

Behren, Andreas ;

Anaka, Matthew ;

Lo, Pu-Han ;

Vella, Laura J. ;

Davis, Ian D. ;

Catimel, Jenny ;

Cardwell, Tracy ;

Gedye, Craig ;

Hudson, Christopher ;

Stan, Rodica ;

Cebon, Jonathan .

PIGMENT CELL & MELANOMA RESEARCH, 2013, 26 (04) :597-600

[2]

CHALMERS AH, 1976, CANCER RES, V36, P1930

[3]

CHENEVIXTRENCH G, 1990, ONCOGENE, V5, P1187

[4] Induction of senescence in diterpene ester-treated melanoma cells via protein kinase C-dependent hyperactivation of the mitogen-activated protein kinase pathway [J].

Cozzi, Sarah-Jane ;

Parsons, Peter G. ;

Ogbourne, Steven M. ;

Pedley, Julie ;

Boyle, Glen M. .

CANCER RESEARCH, 2006, 66 (20) :10083-10091

[5] Melanoma invasion in reconstructed human skin is influenced by skin cells investigation of the role of proteolytic enzymes [J].

Eves, P ;

Katerinaki, E ;

Simpson, C ;

Layton, C ;

Dawson, R ;

Evans, G ;

Mac Neil, S .

CLINICAL & EXPERIMENTAL METASTASIS, 2003, 20 (08) :685-700

[6]

GOSS P, 1977, CANCER RES, V37, P152

[7]

Guo RF, 2013, INT J CLIN EXP PATHO, V6, P1658

[8] Rab7 regulates maturation of melanosomal matrix protein gp100/Pmel17/Silv [J].

Kawakami, Akinori ;

Sakane, Fumio ;

Imai, Shin-Ichi ;

Yasuda, Satoshi ;

Kai, Masahiro ;

Kanoh, Hideo ;

Jin, Hai-Ying ;

Hirosaki, Kuninori ;

Yamashita, Toshiharu ;

Fisher, David E. ;

Jimbow, Kowichi .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2008, 128 (01) :143-150

[9]

Kim Ji Eun, 2013, Frontiers in Genetics, V4, P97, DOI 10.3389/fgene.2013.00097

[10] A Tumor Suppressor Function for the Lipid Phosphatase INPP4B in Melanocytic Neoplasms [J].

Perez-Lorenzo, Rolando ;

Gill, Kamraan Z. ;

Shen, Che-Hung ;

Zhao, Feng X. ;

Zheng, Bin ;

Schulze, Hans-Joachim ;

Silvers, David N. ;

Brunner, Georg ;

Horst, Basil A. .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2014, 134 (05) :1359-1368

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