Cancer stem-like cells in human prostate carcinoma cells DU145 - The seeds of the cell line?

被引：81

作者：

Wei, Chen

Wang, Guomin ^{[1
]}

Liu, Yujun

Qian, Ruizhe

机构：

[1] Fudan Univ, Zhongshan Hosp, Dept Urol, Shanghai 200433, Peoples R China

[2] Fudan Univ, Shanghai Med Coll, Dept Physiol & Pathophysiol, Shanghai 200433, Peoples R China

来源：

CANCER BIOLOGY & THERAPY | 2007年 / 6卷 / 05期

关键词：

cancer stem cells; Du145; prostate cancer; differentiation; CD133; self renew; CD117; CD44; integrin alpha 2 beta 1;

D O I：

10.4161/cbt.6.5.3996

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The aim of this study is to investigate cancer stem cells and their markers in the prostate cancer cell line Du145. Different populations of cells were isolated from Du145. The clones formed by CD44(+) integrin alpha(2)beta(1) (+)CD133(+) cells are remarkably different morphologically and quantitatively from those formed by integrin alpha(2)beta(1) (-/low)CD133(-) cells. CD133(+) cells have the capacity for self renewal, extensive differentiation potential, and high proliferative and tumorigenic potential. CD34(+) and CD117(+) cells have no stem cell properties. Expression levels of c-myc and beta-catenin were elevated and bax was down regulated in CD44(+) integrin alpha(2)beta(1) (+)CD133(+) cells. In summary, CD44, integrin alpha(2)beta(1) and CD133 could be the cancer stem cell makers for the Du145 cell line. CD133(+) cells differed significantly from CD44(+)integrin alpha(2)beta(1) (-/low)CD133(-) cells and the total population in clone generation, genes expression, differentiation, proliferative and tumorigenic potential.

引用

页码：763 / 768

页数：6

共 23 条

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