Serotonin is essential for eye regeneration in planaria Schmidtea mediterranea

被引：10

作者：

Sarkar, Arunabha ^{[1
]}

Mukundan, Namita ^{[2
,3
]}

Sowndarya, Sai ^{[2
]}

Dubey, Vinay Kumar ^{[2
,3
]}

Babu, Rosana ^{[2
]}

Lakshmanan, Vairavan ^{[2
]}

Rangiah, Kannan ^{[4
]}

Panicker, Mitradas M. ^{[1
]}

Palakodeti, Dasaradhi ^{[2
]}

Subramanian, Sabarinath Peruvemba ^{[2
]}

Subramanian, Ramaswamy ^{[2
]}

机构：

[1] Natl Ctr Biol Sci, Bangalore, Karnataka, India

[2] Inst Stem Cell Sci & Regenerat Med InStem, Bangalore 560065, Karnataka, India

[3] Manipal Acad Higher Educ, Manipal, Karnataka, India

[4] Cent Food Technol Res Inst, Mysore, Karnataka, India

来源：

FEBS LETTERS | 2019年 / 593卷 / 22期

关键词：

eye regeneration; LC-MS; phenylalanine hydroxylase; planaria; serotonin; STEM-CELLS; MAINTENANCE; NEUROTRANSMITTERS; MORPHOGENESIS; TRYPTOPHAN; NEOBLASTS; NEURONS; INTACT; GROWTH; GENE;

D O I：

10.1002/1873-3468.13607

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Planaria is an ideal system to study factors involved in regeneration and tissue homeostasis. Little is known about the role of metabolites and small molecules in stem cell maintenance and lineage specification in planarians. Using liquid chromatography and mass spectrometry (LC-MS)-based quantitative metabolomics, we determined the relative levels of metabolites in stem cells, progenitors, and differentiated cells of the planarian Schmidtea mediterranea. Tryptophan and its metabolic product serotonin are significantly enriched in stem cells and progenitor population. Serotonin biosynthesis in these cells is brought about by a noncanonical enzyme, phenylalanine hydroxylase. Knockdown of Smed-pah leads to complete disappearance of eyes in regenerating planaria, while exogenous supply of serotonin and its precursor rescues the eyeless phenotype. Our results demonstrate a key role for serotonin in eye regeneration.

引用

页码：3198 / 3209

页数：12

共 46 条

[1] Phenylalanine hydroxylase participation in the synthesis of serotonin and pteridines in Drosophila melanogaster [J].