BRANCHED-CHAIN AMINO ACIDS PROTECT AGAINST DEXAMETHASONE-INDUCED SOLEUS MUSCLE ATROPHY IN RATS

被引：79

作者：

Yamamoto, Daisuke ^{[1
]}

Maki, Taiki ^{[1
]}

Herningtyas, Elizabeth Henny ^{[1
,2
]}

Ikeshita, Nobuko ^{[1
]}

Shibahara, Hiromi ^{[1
]}

Sugiyama, Yuka ^{[1
]}

Nakanishi, Shiho ^{[1
]}

Iida, Keiji ^{[3
]}

Iguchi, Genzo ^{[3
]}

Takahashi, Yutaka ^{[2
]}

Kaji, Hidesuke ^{[4
]}

Chihara, Kazuo ^{[3
]}

Okimura, Yasuhiko ^{[1
]}

机构：

[1] Kobe Univ, Grad Sch Hlth Sci, Dept Biophys, Suma Ku, Kobe, Hyogo 6540142, Japan

[2] Kobe Univ, Grad Sch Med, Dept Internal Med, Div Endocrinol Diabet & Metab, Kobe, Hyogo 6540142, Japan

[3] Hyogo Prefectural Kakogawa Hosp, Dept Med, Kakogawa, Japan

[4] Univ Hyogo, Coll Nursing Art & Sci, Akashi, Hyogo, Japan

来源：

MUSCLE & NERVE | 2010年 / 41卷 / 06期

关键词：

atrogin-1; autophagy; BCAA; dexamethasone; muscle atrophy; SKELETAL-MUSCLE; FIBER-TYPE; POSTABSORPTIVE RATS; UBIQUITIN LIGASES; GENE-EXPRESSION; IN-VIVO; LEUCINE; AUTOPHAGY; SLOW; PROTEOLYSIS;

D O I：

10.1002/mus.21621

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

We investigated the utility of branched-chain amino acids (BCAA) in dexamethasone-induced muscle atrophy. Dexamethasone (600 mu g/kg, intraperitoneally) and/or BCAA (600 mg/kg, orally) were administered for 5 days in rats, and the effect of BCAA on dexamethasone-induced muscle atrophy was evaluated. Dexamethasone decreased total protein concentration of rat soleus muscles. Concomitant administration of BCAA reversed the decrease. Dexamethasone decreased mean cross-sectional area of soleus muscle fibers, which was reversed by BCAA. Dexamethasone increased atrogin-1 expression, which has been reported to play a pivotal role in muscle atrophy. The increased expression of atrogin-1 mRNA was significantly attenuated by BCAA. Furthermore, dexamethasone-induced conversion from microtubule-associated protein 1 light chain 3 (LC3)-I to LC3-II, which is an indicator of autophagy, was blocked by BCAA. These findings suggest that BCAA decreased protein breakdown to prevent muscle atrophy. BCAA administration appears to be useful for prevention of steroid myopathy. Muscle Nerve 41: 819-827, 2010

引用

页码：819 / 827

页数：9

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