Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa

被引：35

作者：

Calamera, Juan C. ^{[3
]}

Buffone, Mariano G. ^{[4
]}

Doncel, Gustavo F. ^{[5
]}

Brugo-Olmedo, Santiago ^{[6
]}

de Vincentiis, Sabrina ^{[6
]}

Calamera, Maria M. ^{[3
]}

Storey, Bayard T. ^{[4
]}

Alvarez, Juan G. ^{[1
,2
]}

机构：

[1] Inst Marques, Barcelona 08034, Spain

[2] Fdn Leonardo Marques, Barcelona, Spain

[3] Lab Estudios Reprod, Buenos Aires, DF, Argentina

[4] Univ Penn, Ctr Res Reprod & Womens Hlth, Philadelphia, PA 19104 USA

[5] Eastern Virginia Med Sch, Jones Inst Reprod Med, Dept Obstet & Gynecol, Contracept Res & Dev CONRAD, Norfolk, VA USA

[6] Ctr Med Seremas, Buenos Aires, DF, Argentina

来源：

FERTILITY AND STERILITY | 2010年 / 93卷 / 03期

关键词：

Cryopreservation; human sperm; temperature; ATP; DNA damage; SPONTANEOUS LIPID-PEROXIDATION; IN-VITRO FERTILIZATION; SPERM STRESS TEST; SEMINAL PLASMA; SUPEROXIDE-DISMUTASE; ASSAY; MEMBRANE; PREDICTS; DAMAGE; PHOSPHOLIPIDS;

D O I：

10.1016/j.fertnstert.2008.10.021

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To investigate the effects of thawing temperature oil sperm function after cryopreservation. The technical aspects of sperm cryopreservation have significantly improved over the last few decades. However, a standard protocol designed to optimize sperm motility recovery after thawing has not yet been established. Design: Prospective study. Setting: Private infertility institute and university-based research laboratory. Patient(s): Eighty consenting normozoospermic patients consulting for infertility. Intervention(s): Spermatozoa From donor semen samples were thawed at different temperatures. Main Outcome Measure(s): Sperm motility. viability, adenosine-5'-triphosphate (ATP) content, acrosomal status, and DNA integrity were evaluated as a function of thawing temperature in cryopreserved human sperm samples. Result(s): Thawing at 40 degrees C resulted in a statistically significant increase in sperm motility recovery compared with thawing at temperatures between 20 degrees C and 37 degrees C. There were no statistically significant differences in sperm viability, acrosomal status, ATP content. and DNA integrity after thawing at 40 degrees C compared with thawing at temperatures between 20 degrees C and 37 degrees C. Conclusion(s): Sperm thawing at 40 degrees C could be safely used to improve motility recovery after sperm cryopreservation. (Fertil Steril (R) 220 10;93:789-94. (C)2010 by American Society for Reproductive Medicine.)

引用

页码：789 / 794

页数：6

共 31 条

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