Short telomeres induce a DNA damage response in Saccharomyces cerevisiae

被引：146

作者：

Ijpma, AS ^{[1
]}

Greider, CW ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, Grad Program Cellular & Mol Med, Baltimore, MD 21205 USA

来源：

MOLECULAR BIOLOGY OF THE CELL | 2003年 / 14卷 / 03期

关键词：

D O I：

10.1091/mbc.02-04-0057

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Telomerase-deficient Saccharomyces cerevisiae cells show a progressive decrease in telomere length. When grown for several days in log phase, the tlc1Delta cells initially display wild-type growth kinetics with subsequent loss of growth potential after which survivors are generated via RAD52-dependent homologous recombination. We found that chromosome loss in these telomerase-deficient cells only increased after a significant decline in growth potential of the culture. At earlier stages of growth, as the telomerase-deficient cells began to show loss of growth potential, the cells arrested in G2/M and showed RNR3 induction and Rac53p phosphorylation. These responses were dependent on RAD24 and MECL suggesting that short telomeres are recognized as DNA damage and signal G2/M arrest.

引用

页码：987 / 1001

页数：15

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