Electrospun 3D Fibrous Scaffolds for Chronic Wound Repair

被引:66
作者
Chen, Huizhi [1 ,2 ]
Peng, Yan [3 ]
Wu, Shucheng [3 ]
Tan, Lay Poh [1 ]
机构
[1] Nanyang Technol Univ, Sch Mat Sci & Engn, 50 Nanyang Ave, Singapore 639798, Singapore
[2] Nanyang Technol Univ, Interdisciplinary Grad Sch, 50 Nanyang Ave, Singapore 639798, Singapore
[3] Ngee Ann Polytech, Sch Mech Engn, 535 Clementi Rd, Singapore 599489, Singapore
关键词
electrospinning; tissue engineering; wound healing; PLGA; fibrous scaffolds; surface modification; SURFACE MODIFICATION; CELL INFILTRATION; DRUG-DELIVERY; TISSUE; NANOFIBERS; IMMOBILIZATION; ATTACHMENT; MEMBRANE; POLYMERS; COLLAGEN;
D O I
10.3390/ma9040272
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Chronic wounds are difficult to heal spontaneously largely due to the corrupted extracellular matrix (ECM) where cell ingrowth is obstructed. Thus, the objective of this study was to develop a three-dimensional (3D) biodegradable scaffold mimicking native ECM to replace the missing or dysfunctional ECM, which may be an essential strategy for wound healing. The 3D fibrous scaffolds of poly(lactic acid-co-glycolic acid) (PLGA) were successfully fabricated by liquid-collecting electrospinning, with 5 similar to 20 mu m interconnected pores. Surface modification with the native ECM component aims at providing biological recognition for cell growth. Human dermal fibroblasts (HDFs) successfully infiltrated into scaffolds at a depth of similar to 1400 mu m after seven days of culturing, and showed significant progressive proliferation on scaffolds immobilized with collagen type I. In vivo models showed that chronic wounds treated with scaffolds had a faster healing rate. These results indicate that the 3D fibrous scaffolds may be a potential wound dressing for chronic wound repair.
引用
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页数:12
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