A simple and objective approach to identifying human round spermatids

被引：32
作者：
Angelopoulos, T
Krey, L
McCullough, A
Adler, A
Grifo, JA
机构：
[1] NYU, Med Ctr, Dept Obstet & Gynecol, New York, NY 10016 USA
[2] NYU, Med Ctr, Dept Urol, New York, NY 10016 USA
来源：
HUMAN REPRODUCTION | 1997年 / 12卷 / 10期
关键词：
fluorescence in-situ hybridization; non-obstructive azoospermia; round spermatids; spermatogenic arrest; testicular biopsy;
D O I：
10.1093/humrep/12.10.2208
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
Although round spermatids have been studied extensively using staining techniques and electron microscopy, little information is available about their appearance in living conditions. We describe a method of collecting and identifying round spermatids from ejaculates and testicular biopsies. The validity of the selection procedure was confirmed, by fluorescence in-situ hybridization. Based on cell size, morphological characteristics of nucleus and cytoplasm, and on the nucleus/cytoplasm ratio, we harvested a population of cells that was 84% haploid. This procedure can be applied to select spermatids for clinical or research purposes.
引用
收藏
页码：2208 / 2216
页数：9
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