The Effects and Mechanism of miR-92a and miR-126 on Myocardial Apoptosis in Mouse Ischemia-Reperfusion Model

被引:28
作者
Jiang, Changhao [1 ]
Ji, Ningning [1 ]
Luo, Gaojiang [1 ]
Ni, Shimao [1 ]
Zong, Jinbo [1 ]
Chen, Zhili [1 ]
Bao, Donglai [1 ]
Gong, Xinyan [1 ]
Fu, Ting [1 ]
机构
[1] Wenzhou Med Univ, Yiwu Cent Hosp, Yiwu Hosp, Dept Cardiol, Yiwu 322000, Zhejiang, Peoples R China
关键词
Ischemia-reperfusion; Myocardial cell; Apoptosis; HSP70; miR-92a; miR-126; ISCHEMIA/REPERFUSION INJURY; EXPRESSION; INFARCTION; DISEASE; GROWTH; CELLS;
D O I
10.1007/s12013-014-0149-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Our objective was to explore the effects of miR-92a and miR-126 on myocardial apoptosis in mouse ischemia-reperfusion model and further investigate the underlying mechanisms. Eighteen Kunming mice were selected and randomly divided into sham operation group and ischemia-reperfusion group with nine mice in each group. Cardiac muscle tissue was stained with Evans blue to confirm myocardial infarction and ischemia. Annexin V/PI double staining was used to detect the apoptotic rate of myocardial cells, and terminal-deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) was used to detect the number of apoptotic cells; Western blot was used to detect expression of Caspase 3 to evaluate the apoptosis of mouse myocardial cells; qRT-PCR was used to detect expression of miR-92a and miR-126 in mouse myocardium, and Western blot was used to detect expression of HSP70 in two groups. Evans blue staining results showed that there was a large area of ischemia in myocardium of ischemia-reperfusion mice with marked infarction, suggesting successful establishment of the model. In sham operation group, myocardial cells were mostly normal cells. Annexin V/PI double staining of flow cytometry result showed that the apoptotic rate was 5.9 % in sham operation group and 37.0 % in ischemia-reperfusion group, respectively. Apoptosis detection results showed that apoptotic index (AI) of myocardial cells in ischemia-reperfusion mice was significantly higher than in sham operation group. In addition, qRT-PCR results showed that miR-92a expression in ischemia-reperfusion group was significantly higher than in sham operation group (F = 32.302, P = 0.000), and miR-126 expression in ischemia-reperfusion group was significantly lower than in sham operation group (F = 41.125, P = 0.000). Moreover, HSP70 detected by Western blot showed that HSP expression in ischemia-reperfusion group was significantly lower than in sham operation group. The change of miR-92a was in accordance with AI of myocardial cells. However, the change of miR-126 is in contrary with AI of myocardial cells, which may be related to the HSP70 expression in myocardial cells.
引用
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页码:1901 / 1906
页数:6
相关论文
共 34 条
[1]  
Alexandre Joachim, 2013, Int J Physiol Pathophysiol Pharmacol, V5, P52
[2]   Development of a miR-92a delivery system for anti-angiogenesis-based cancer therapy [J].
Ando, Hidenori ;
Okamoto, Ayaka ;
Yokota, Masafumi ;
Shimizu, Kosuke ;
Asai, Tomohiro ;
Dewa, Takehisa ;
Oku, Naoto .
JOURNAL OF GENE MEDICINE, 2013, 15 (01) :20-27
[3]   Pomegranate polyphenolics suppressed azoxymethane-induced colorectal aberrant crypt foci and inflammation: possible role of miR-126/VCAM-1 and miR-126/PI3K/AKT/mTOR [J].
Banerjee, Nivedita ;
Kim, Hyemee ;
Talcott, Stephen ;
Mertens-Talcott, Susanne .
CARCINOGENESIS, 2013, 34 (12) :2814-2822
[4]   Insulin-blood cardioplegia decreases matrix metalloproteinase activity in ischaemia-reperfusion injury during coronary artery bypass surgery [J].
Catalyurek, H. ;
Oktay, G. ;
Guzeloglu, M. ;
Cavdar, Z. ;
Acikel, U. ;
Silistreli, E. ;
Hazan, E. .
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, 2008, 36 (03) :551-558
[5]   Rho-kinase Levels in Testicular Ischemia-reperfusion Injury and Effects of Its Inhibitor, Y-27632, on Oxidative Stress, Spermatogenesis, and Apoptosis [J].
Cayan, Selahittin ;
Saylam, Baris ;
Tiftik, Nalan ;
Unal, Nil Dogruer ;
Apa, Duygu Dusmez ;
Efesoy, Ozan ;
Cimen, Burak ;
Bozlu, Murat ;
Akbay, Erdem ;
Buyukafsar, Kansu .
UROLOGY, 2014, 83 (03) :675.e13-675.e18
[6]   MicroRNA Signatures in Ischemia-Reperfusion Injury [J].
Chang, Kao-Ping ;
Lee, Hsiao-Chen ;
Huang, Shu-Hung ;
Lee, Su-Shin ;
Lai, Chung-Sheng ;
Lin, Sin-Daw .
ANNALS OF PLASTIC SURGERY, 2012, 69 (06) :668-671
[7]   Heparin-binding epidermal growth factor-like growth factor restores Wnt/β-catenin signaling in intestinal stem cells exposed to ischemia/reperfusion injury [J].
Chen, Chun-Liang ;
Yang, Jixin ;
James, Iyore O. A. ;
Zhang, Hong-yi ;
Besner, Gail E. .
SURGERY, 2014, 155 (06) :1069-1080
[8]  
Chen Y, 2013, J INVASIVE CARDIOL, V25, P376
[9]   Estrogens protect myocardium against ischemia/reperfusion insult by up-regulation of CRH receptor type 2 in female rats [J].
Cong, Binhai ;
Zhu, Xiaoyan ;
Cao, Buqing ;
Xiao, Jian ;
Wang, Zhinong ;
Ni, Xin .
INTERNATIONAL JOURNAL OF CARDIOLOGY, 2013, 168 (05) :4755-4760
[10]   Letter by D'Alessandra et al Regarding Article, "Circulating MicroRNA-208b and MicroRNA-499 Reflect Myocardial Damage in Cardiovascular Disease" [J].
D'Alessandra, Yuri ;
Pompilio, Giulio ;
Capogrossi, Maurizio C. .
CIRCULATION-CARDIOVASCULAR GENETICS, 2011, 4 (01) :E7-E7